8C4T

Hantaan virus polymerase bound to its 5' viral RNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	RNA-directed RNA polymerase L	polymer	2173	249432.5	1	UniProt (P23456) Pfam (PF21991) Pfam (PF12426) Pfam (PF04196)	Hantaan virus 76-118	Protein L,Large structural protein,RdRp,Replicase,Transcriptase
2	B (C)	RNA (5'-R(P*UP*AP*GP*UP*AP*GP*UP*AP*GP*AP*CP*A)-3')	polymer	20	6453.9	1		Hantaan virus 76-118
3	C (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

A: 1 - 2151 (UniProt: P23456)

PDB	External Database	Details
Met -21	-	initiating methionine
Gly -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
Asp -13	-	expression tag
Tyr -12	-	expression tag
Asp -11	-	expression tag
Ile -10	-	expression tag
Pro -9	-	expression tag
Thr -8	-	expression tag
Thr -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Ala 97	Asp 97	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	255886.4
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	255910.7

*Water molecules are not included.