8C49
Crystal structure of pyrrolysyl-tRNA synthetase from Methanomethylophilus alvus engineered for 3-Methyl-L-histidine, bound to AMPPNP
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Pyrrolysyl-tRNA synthetase | polymer | 283 | 31859.1 | 2 | UniProt (M9SC49) Pfam (PF17759) In PDB | Candidatus Methanomethylophilus alvus | |
2 | A, B | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 2 | Chemie (ANP) | |||
3 | A, B | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 7 | Chemie (PEG) | |||
4 | A, B | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 3 | Chemie (PGE) | |||
5 | A, B | 1,2-ETHANEDIOL | non-polymer | 62.1 | 4 | Chemie (EDO) | |||
6 | B, A | MAGNESIUM ION | non-polymer | 24.3 | 11 | Chemie (MG) | |||
7 | water | water | 18.0 | 255 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 275 (UniProt: M9SC49)
PDB | External Database | Details |
---|---|---|
Ile 125 | Leu 125 | engineered mutation |
Phe 126 | Tyr 126 | engineered mutation |
Gly 129 | Met 129 | engineered mutation |
Phe 168 | Val 168 | engineered mutation |
Phe 206 | Tyr 206 | engineered mutation |
Leu 276 | - | expression tag |
Glu 277 | - | expression tag |
His 278 | - | expression tag |
His 279 | - | expression tag |
His 280 | - | expression tag |
His 281 | - | expression tag |
His 282 | - | expression tag |
His 283 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 63718.3 | |
Non-Polymers* | Number of molecules | 27 |
Total formula weight | 2721.4 | |
All* | Total formula weight | 66439.6 |