8C42

Ternary structure of 14-3-3sigma, PKA-responsive ERa phosphopeptide and Fusicoccin-A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	14-3-3 protein sigma	polymer	236	26542.9	1	UniProt (P31947) Pfam (PF00244) In PDB	Homo sapiens (human)	Epithelial cell marker protein 1,Stratifin
2	B	Estrogen receptor	polymer	8	940.9	1	UniProt (P03372) In PDB	Homo sapiens (human)	ER,ER-alpha,Estradiol receptor,Nuclear receptor subfamily 3 group A member 1
3	A	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
4	A	De-acetylated Fusicoccin	non-polymer		638.8	1	Chemie (SIT)
5		water	water		18.0	267	Chemie (HOH)

Sequence modifications

A: 1 - 231 (UniProt: P31947)

PDB	External Database	Details
Gly -4	-	expression tag
Ala -3	-	expression tag
Met -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

B: 588 - 595 (UniProt: P03372)

PDB	External Database	Details
Arg 591	Phe 591	engineered mutation
Arg 592	Pro 592	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	27483.9
Non-Polymers*	Number of molecules	4
	Total formula weight	711.7
All*	Total formula weight	28195.6

*Water molecules are not included.