8BXZ

Crystal structure of Paradendryphiella salina PL7C alginate lyase mutant Y220F in complex with penta-mannuronic acid

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Alginate lyase	polymer	234	25528.2	1	UniProt (A0A7I9C8Z1) Pfam (PF08787) In PDB	Paradendryphiella salina
2	B	beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid	branched		898.6	1	In PDB GlyTouCan (G61688UV)
3	A	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
4		water	water		18.0	382	Chemie (HOH)

Sequence modifications

A: 17 - 240 (UniProt: A0A7I9C8Z1)

PDB	External Database	Details
Glu -1	-	expression tag
Phe 0	-	expression tag
Phe 220	Tyr 220	engineered mutation
Val 241	-	expression tag
Asp 242	-	expression tag
His 243	-	expression tag
His 244	-	expression tag
His 245	-	expression tag
His 246	-	expression tag
His 247	-	expression tag
His 248	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	25528.2
Branched	Number of molecules	1
	Total formula weight	898.6
Non-Polymers*	Number of molecules	1
	Total formula weight	96.1
All*	Total formula weight	26522.9

*Water molecules are not included.