8BS0

Room-temperature structure of Pedobacter heparinus N-acetylglucosamine 2-epimerase at 80 MPa helium gas pressure in a sapphire capillary

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	N-acylglucosamine 2-epimerase	polymer	418	48868.9	2	UniProt (C6Y403) Pfam (PF07221)	Pedobacter heparinus
2	C, E (A, B)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
3	D, F (A, B)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
4	G, H (A, B)	water	water		18.0	421	Chemie (HOH)

Sequence modifications

A, B: 2 - 403 (UniProt: C6Y403)

PDB	External Database	Details
Met -14	-	initiating methionine
Gly -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
Ser -4	-	expression tag
Gln -3	-	expression tag
Asp -2	-	expression tag
Pro -1	-	expression tag
Asn 0	-	expression tag
Ser 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	97737.9
Non-Polymers*	Number of molecules	4
	Total formula weight	260.8
All*	Total formula weight	97998.7

*Water molecules are not included.