8BRX

Escherichia coli methionyl-tRNA synthetase mutant L13C,I297C complexed with beta-3-methionine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Methionine--tRNA ligase	polymer	568	64710.0	1	UniProt (P00959) Pfam (PF09334) Pfam (PF19303) In PDB	Escherichia coli	Methionyl-tRNA synthetase,MetRS
2	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
3	A	CITRIC ACID	non-polymer		192.1	2	Chemie (CIT)
4	A	(3R)-3-amino-5-(methylsulfanyl)pentanoic acid	non-polymer		163.2	1	Chemie (B3M)
5		water	water		18.0	638	Chemie (HOH)

Sequence modifications

A: 1 - 547 (UniProt: P00959)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Ser 0	-	expression tag
Cys 13	Leu 14	engineered mutation
Cys 297	Ile 298	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	64710.0
Non-Polymers*	Number of molecules	4
	Total formula weight	612.9
All*	Total formula weight	65322.9

*Water molecules are not included.