8BRR
Crystal structure of a variant of penicillin G acylase from Bacillaceae i. s. sp. FJAT-27231 with reduced surface entropy and additionally engineered crystal contact
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, C | Penicillin G acylase | polymer | 212 | 24683.8 | 2 | UniProt (A0A0K9H482) In PDB | Bacillus sp. FJAT-27231 | |
2 | B, D | Penicillin G acylase | polymer | 538 | 61372.8 | 2 | UniProt (A0A0K9H482) Pfam (PF01804) In PDB | Bacillus sp. FJAT-27231 | |
3 | B, D | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 2 | Chemie (EPE) | |||
4 | B, D | (R,R)-2,3-BUTANEDIOL | non-polymer | 90.1 | 2 | Chemie (BU3) | |||
5 | B, D | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
6 | water | water | 18.0 | 1109 | Chemie (HOH) |
Sequence modifications
A, C: 1 - 212 (UniProt: A0A0K9H482)
B, D: 1 - 538 (UniProt: A0A0K9H482)
PDB | External Database | Details |
---|---|---|
Ala 201 | Lys 225 | engineered mutation |
Ala 202 | Lys 226 | engineered mutation |
Ala 203 | Glu 227 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Ala 135 | Lys 402 | engineered mutation |
Ala 136 | Glu 403 | engineered mutation |
Ala 137 | Lys 404 | engineered mutation |
Ala 403 | Lys 670 | engineered mutation |
Ala 404 | Glu 671 | engineered mutation |
Ala 405 | Glu 672 | engineered mutation |
Val 408 | Lys 675 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 172113.3 | |
Non-Polymers* | Number of molecules | 8 |
Total formula weight | 817.2 | |
All* | Total formula weight | 172930.4 |