8BRQ
Crystal structure of a surface entropy reduction variant of penicillin G acylase from Bacillaceae i. s. sp. FJAT-27231
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Penicillin G acylase | polymer | 212 | 24683.8 | 1 | UniProt (A0A0K9H482) | Bacillus sp. FJAT-27231 | |
| 2 | B (B) | Penicillin G acylase | polymer | 538 | 61402.9 | 1 | UniProt (A0A0K9H482) Pfam (PF01804) | Bacillus sp. FJAT-27231 | |
| 3 | C (B) | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 1 | Chemie (EPE) | |||
| 4 | D, E, F (B) | (R,R)-2,3-BUTANEDIOL | non-polymer | 90.1 | 3 | Chemie (BU3) | |||
| 5 | G, H (B) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 6 | I, J (A, B) | water | water | 18.0 | 860 | Chemie (HOH) |
Sequence modifications
A: 1 - 212 (UniProt: A0A0K9H482)
B: 1 - 538 (UniProt: A0A0K9H482)
| PDB | External Database | Details |
|---|---|---|
| Ala 201 | Lys 225 | engineered mutation |
| Ala 202 | Lys 226 | engineered mutation |
| Ala 203 | Glu 227 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ala 135 | Lys 402 | engineered mutation |
| Ala 136 | Glu 403 | engineered mutation |
| Ala 137 | Lys 404 | engineered mutation |
| Ala 403 | Lys 670 | engineered mutation |
| Ala 404 | Glu 671 | engineered mutation |
| Ala 405 | Glu 672 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 86086.7 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 588.8 | |
| All* | Total formula weight | 86675.5 |
