8BQH

W-formate dehydrogenase from Desulfovibrio vulgaris - Soaking with Formate 1.5 min

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Formate dehydrogenase, alpha subunit, selenocysteine-containing	polymer	1013	112437.0	1	UniProt (Q72EJ1) Pfam (PF04879) Pfam (PF00384) Pfam (PF01568)	Desulfovibrio vulgaris str. Hildenborough
2	B (B)	Formate dehydrogenase, beta subunit, putative	polymer	236	26481.5	1	UniProt (Q72EJ0) Pfam (PF13247)	Desulfovibrio vulgaris str. Hildenborough
3	C, D (A)	2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE GUANOSINE DINUCLEOTIDE	non-polymer		740.6	2	Chemie (MGD)
4	CA, DA, E, EA (B, A)	IRON/SULFUR CLUSTER	non-polymer		351.6	4	Chemie (SF4)
5	F (A)	HYDROSULFURIC ACID	non-polymer		34.1	1	Chemie (H2S)
6	G (A)	TUNGSTEN ION	non-polymer		183.8	1	Chemie (W)
7	H, I (A)	TETRAETHYLENE GLYCOL	non-polymer		194.2	2	Chemie (PG4)
8	J, K (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
9	L, M, Q, S (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	4	Chemie (PEG)
10	N, O, P, R (A)	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
11	FA, T, U, V, W (B, A)	FORMIC ACID	non-polymer		46.0	5	Chemie (FMT)
12	AA, BA, X, Y, Z (A)	CHLORIDE ION	non-polymer		35.5	5	Chemie (CL)
13	GA, HA (A, B)	water	water		18.0	557	Chemie (HOH)

Sequence modifications

A: 36 - 1005 (UniProt: Q72EJ1)

PDB	External Database	Details
Met 1	-	initiating methionine
Thr 2	-	expression tag
Val 3	-	expression tag
Thr 4	-	expression tag
Arg 5	-	expression tag
Arg 6	-	expression tag
His 7	-	expression tag
Phe 8	-	expression tag
Leu 9	-	expression tag
Lys 10	-	expression tag
Leu 11	-	expression tag
Ser 12	-	expression tag
Ala 13	-	expression tag
Gly 14	-	expression tag
Ala 15	-	expression tag
Ala 16	-	expression tag
Val 17	-	expression tag
Ala 18	-	expression tag
Gly 19	-	expression tag
Ala 20	-	expression tag
Phe 21	-	expression tag
Thr 22	-	expression tag
Gly 23	-	expression tag
Leu 24	-	expression tag
Gly 25	-	expression tag
Leu 26	-	expression tag
Ser 27	-	expression tag
Leu 28	-	expression tag
Ala 29	-	expression tag
Pro 30	-	expression tag
Thr 31	-	expression tag
Val 32	-	expression tag
Ala 33	-	expression tag
Arg 34	-	expression tag
Ala 35	-	expression tag
Trp 1006	-	expression tag
Ser 1007	-	expression tag
His 1008	-	expression tag
Pro 1009	-	expression tag
Gln 1010	-	expression tag
Phe 1011	-	expression tag
Glu 1012	-	expression tag
Lys 1013	-	expression tag

B: 2 - 215 (UniProt: Q72EJ0)

PDB	External Database	Details
Met 1	-	initiating methionine
Asp 216	-	expression tag
Leu 217	-	expression tag
Ala 218	-	expression tag
Pro 219	-	expression tag
Ser 220	-	expression tag
Met 221	-	expression tag
Met 222	-	expression tag
Thr 223	-	expression tag
Arg 224	-	expression tag
Gln 225	-	expression tag
Gln 226	-	expression tag
Leu 227	-	expression tag
Phe 228	-	expression tag
Ala 229	-	expression tag
Arg 230	-	expression tag
Leu 231	-	expression tag
Phe 232	-	expression tag
Arg 233	-	expression tag
Pro 234	-	expression tag
Arg 235	-	expression tag
Ala 236	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	138918.5
Non-Polymers*	Number of molecules	30
	Total formula weight	4758.4
All*	Total formula weight	143676.9

*Water molecules are not included.