8BPN

The structure of thiocyanate dehydrogenase mutant form with Phe 436 replaced by Gln from Thioalkalivibrio paradoxus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Twin-arginine translocation signal domain-containing protein	polymer	494	54983.7	4	UniProt (W0DP94)	Thioalkalivibrio paradoxus ARh 1
2	E, H (A, B)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
3	F, G, I, J, K... (A, B, C, D)	COPPER (II) ION	non-polymer		63.5	8	Chemie (CU)
4	O, P, Q, R (A, B, C, D)	water	water		18.0	424	Chemie (HOH)

Sequence modifications

A, B, C, D: 82 - 548 (UniProt: W0DP94)

PDB	External Database	Details
Met 55	-	initiating methionine
Ser 56	-	expression tag
Tyr 57	-	expression tag
Tyr 58	-	expression tag
His 59	-	expression tag
His 60	-	expression tag
His 61	-	expression tag
His 62	-	expression tag
His 63	-	expression tag
His 64	-	expression tag
Asp 65	-	expression tag
Tyr 66	-	expression tag
Asp 67	-	expression tag
Ile 68	-	expression tag
Pro 69	-	expression tag
Thr 70	-	expression tag
Thr 71	-	expression tag
Glu 72	-	expression tag
Asn 73	-	expression tag
Leu 74	-	expression tag
Tyr 75	-	expression tag
Phe 76	-	expression tag
Gln 77	-	expression tag
Gly 78	-	expression tag
Ala 79	-	expression tag
Met 80	-	expression tag
Gly 81	-	expression tag
Gln 436	Phe 436	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	219934.6
Non-Polymers*	Number of molecules	10
	Total formula weight	720.6
All*	Total formula weight	220655.2

*Water molecules are not included.