8BNZ

BAM-EspP complex structure with BamA-G431C/EspP-N1293C mutations in nanodisc

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Outer membrane protein assembly factor BamA	polymer	810	90689.5	1	UniProt (P0A940) Pfam (PF07244) Pfam (PF01103)	Escherichia coli K-12	Omp85
2	B (B)	Outer membrane protein assembly factor BamB	polymer	392	41918.9	1	UniProt (P77774) Pfam (PF01011) Pfam (PF13360)	Escherichia coli K-12
3	C (C)	Outer membrane protein assembly factor BamC	polymer	344	36875.3	1	UniProt (P0A903) Pfam (PF06804)	Escherichia coli K-12
4	D (D)	Outer membrane protein assembly factor BamD	polymer	245	27858.3	1	UniProt (P0AC02) Pfam (PF13525)	Escherichia coli K-12
5	E (E)	Outer membrane protein assembly factor BamE	polymer	123	13530.3	1	UniProt (P0A937) Pfam (PF04355)	Escherichia coli K-12
6	F (P)	Serine protease EspP	polymer	353	38727.8	1	UniProt (Q7BSW5) Pfam (PF03797)	Escherichia coli O157:H7

Sequence modifications

A: 1 - 810 (UniProt: P0A940)

PDB	External Database	Details
Cys 431	Gly 431	engineered mutation

E: 1 - 113 (UniProt: P0A937)

PDB	External Database	Details
Gly 114	-	expression tag
Gly 115	-	expression tag
His 116	-	expression tag
His 117	-	expression tag
His 118	-	expression tag
His 119	-	expression tag
His 120	-	expression tag
His 121	-	expression tag
His 122	-	expression tag
His 123	-	expression tag

P: 948 - 1300 (UniProt: Q7BSW5)

PDB	External Database	Details
Cys 1293	Asn 1293	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	249600.1
All*	Total formula weight	249600.1

*Water molecules are not included.