8BMS

Cryo-EM structure of the mutant solitary ECF module 2EQ in MSP2N2 lipid nanodiscs in the ATPase closed and ATP-bound conformation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Energy-coupling factor transporter ATP-binding protein EcfA1	polymer	282	30800.9	1	UniProt (Q1GBJ0) Pfam (PF00005) In PDB	Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002	ECF transporter A component EcfA1
2	B	Energy-coupling factor transporter ATP-binding protein EcfA2	polymer	287	31671.2	1	UniProt (Q1GBI9) Pfam (PF00005) In PDB	Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002	ECF transporter A component EcfA2
3	C	Energy-coupling factor transporter transmembrane protein EcfT	polymer	265	30290.3	1	UniProt (Q1GBI8) Pfam (PF02361) In PDB	Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002	ECF transporter T component EcfT
4	A, B	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
5	A, B	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6		water	water		18.0	72	Chemie (HOH)

Sequence modifications

A: 2 - 282 (UniProt: Q1GBJ0)

PDB	External Database	Details
Gly 1	-	expression tag
Gln 169	Glu 169	engineered mutation

B: 1 - 287 (UniProt: Q1GBI9)

PDB	External Database	Details
Gln 171	Glu 171	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	92762.3
Non-Polymers*	Number of molecules	4
	Total formula weight	1063.0
All*	Total formula weight	93825.3

*Water molecules are not included.