8BMQ

Cryo-EM structure of the folate-specific ECF transporter complex in MSP2N2 lipid nanodiscs bound to AMP-PNP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Energy-coupling factor transporter ATP-binding protein EcfA1	polymer	300	33166.4	1	UniProt (Q1GBJ0) Pfam (PF00005) In PDB	Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002	ECF transporter A component EcfA1
2	B	Energy-coupling factor transporter ATP-binding protein EcfA2	polymer	287	31672.2	1	UniProt (Q1GBI9) Pfam (PF00005) In PDB	Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002	ECF transporter A component EcfA2
3	C	Folate family ECF transporter S component	polymer	184	20483.6	1	UniProt (Q1G929) Pfam (PF12822) In PDB	Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002	Conserved hypothetical membrane protein
4	D	Energy-coupling factor transporter transmembrane protein EcfT	polymer	265	30290.3	1	UniProt (Q1GBI8) Pfam (PF02361) In PDB	Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002	ECF transporter T component EcfT
5	A, B	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	2	Chemie (ANP)
6	B	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

A: 2 - 282 (UniProt: Q1GBJ0)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Gly -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag
Gly 1	-	expression tag

C: 1 - 176 (UniProt: Q1G929)

PDB	External Database	Details
Trp 177	-	insertion
Ser 178	-	insertion
His 179	-	insertion
Pro 180	-	insertion
Gln 181	-	insertion
Phe 182	-	insertion
Glu 183	-	insertion
Lys 184	-	insertion

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	115612.5
Non-Polymers*	Number of molecules	3
	Total formula weight	1036.7
All*	Total formula weight	116649.2

*Water molecules are not included.