8BMP
Cryo-EM structure of the folate-specific ECF transporter complex in MSP2N2 lipid nanodiscs bound to ATP and ADP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Energy-coupling factor transporter ATP-binding protein EcfA1 | polymer | 300 | 33166.4 | 1 | UniProt (Q1GBJ0) Pfam (PF00005) | Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002 | ECF transporter A component EcfA1 |
| 2 | B (B) | Energy-coupling factor transporter ATP-binding protein EcfA2 | polymer | 287 | 31672.2 | 1 | UniProt (Q1GBI9) Pfam (PF00005) | Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002 | ECF transporter A component EcfA2 |
| 3 | C (C) | Folate family ECF transporter S component | polymer | 184 | 20483.6 | 1 | UniProt (Q1G929) Pfam (PF12822) | Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002 | Conserved hypothetical membrane protein |
| 4 | D (D) | Energy-coupling factor transporter transmembrane protein EcfT | polymer | 265 | 30290.3 | 1 | UniProt (Q1GBI8) Pfam (PF02361) | Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002 | ECF transporter T component EcfT |
| 5 | E (A) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 1 | Chemie (ATP) | |||
| 6 | F (B) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
| 7 | G (B) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
A: 2 - 282 (UniProt: Q1GBJ0)
C: 1 - 176 (UniProt: Q1G929)
| PDB | External Database | Details |
|---|---|---|
| Met -17 | - | initiating methionine |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| Gly -6 | - | expression tag |
| Glu -5 | - | expression tag |
| Asn -4 | - | expression tag |
| Leu -3 | - | expression tag |
| Tyr -2 | - | expression tag |
| Phe -1 | - | expression tag |
| Gln 0 | - | expression tag |
| Gly 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Trp 177 | - | insertion |
| Ser 178 | - | insertion |
| His 179 | - | insertion |
| Pro 180 | - | insertion |
| Gln 181 | - | insertion |
| Phe 182 | - | insertion |
| Glu 183 | - | insertion |
| Lys 184 | - | insertion |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 115612.5 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 958.7 | |
| All* | Total formula weight | 116571.1 |
