8BJO
Crystal structure of Paradendryphiella salina PL7C alginate lyase mutant Y220F in complex with hexa-mannuronic acid
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Alginate lyase | polymer | 234 | 25528.2 | 1 | UniProt (A0A7I9C8Z1) Pfam (PF08787) | Paradendryphiella salina | |
| 2 | B (B) | beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid | branched | 1074.8 | 1 | In PDB GlyTouCan (G52261FN) | |||
| 3 | C (A) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 4 | D (A) | water | water | 18.0 | 217 | Chemie (HOH) |
Sequence modifications
A: 17 - 240 (UniProt: A0A7I9C8Z1)
| PDB | External Database | Details |
|---|---|---|
| Glu -1 | - | expression tag |
| Phe 0 | - | expression tag |
| Phe 220 | Tyr 220 | engineered mutation |
| Val 241 | - | expression tag |
| Asp 242 | - | expression tag |
| His 243 | - | expression tag |
| His 244 | - | expression tag |
| His 245 | - | expression tag |
| His 246 | - | expression tag |
| His 247 | - | expression tag |
| His 248 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 25528.2 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 1074.8 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 96.1 | |
| All* | Total formula weight | 26699.1 |
