8BII

O-Methyltransferase Plu4895 (mutant H229N) in complex with SAH

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	methyltransferase Plu4895 H229N mutant	polymer	335	38411.8	8	UniProt (A0A6L9JQS9) Pfam (PF00891) UniProt (by SIFTS) (Q7MY04) In PDB	Photorhabdus laumondii subsp. laumondii TTO1
2	A, B, C, D, E...	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	8	Chemie (SAH)
3	E	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4		water	water		18.0	52	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H: 1 - 317 (UniProt: A0A6L9JQS9)

PDB	External Database	Details
Met -17	-	initiating methionine
Arg -16	-	expression tag
Gly -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Asn 229	His 229	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	307294.4
Non-Polymers*	Number of molecules	9
	Total formula weight	3110.7
All*	Total formula weight	310405.2

*Water molecules are not included.