8BHS

GABA-A receptor a5 homomer - a5V3 - RO4938581

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E	Gamma-aminobutyric acid receptor subunit alpha-5	polymer	360	41057.9	5	UniProt (P31644) Pfam (PF02931) Pfam (PF02932) In PDB	Homo sapiens (human)	GABA(A) receptor subunit alpha-5
2	A, B, C, D, E	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	5	Chemie (NAG)
3	A, B, C, D, E	5-[bis(fluoranyl)methyl]-15-bromanyl-2,4,8,9,11-pentazatetracyclo[11.4.0.0^{2,6}.0^{8,12}]heptadeca-1(13),3,5,9,11,14,16-heptaene	non-polymer		352.1	5	Chemie (QK9)

Sequence modifications

A, B, C, D, E: 1 - 315 (UniProt: P31644)

PDB	External Database	Details
Met 48	Ile 79	engineered mutation
Asn 51	Thr 82	engineered mutation
Ile 67	Val 98	engineered mutation
Ala 70	Arg 101	engineered mutation
Thr 72	Ser 103	engineered mutation
Phe 111	Ile 142	engineered mutation
Trp 114	Asn 145	engineered mutation
Arg 120	Lys 151	engineered mutation
Met 121	Leu 152	engineered mutation
Ile 124	Leu 155	engineered mutation
Trp 125	Glu 156	engineered mutation
Asn 126	Asp 157	engineered mutation
Arg 129	Thr 160	engineered mutation
Val 130	Leu 161	engineered mutation
Leu 134	Met 165	engineered mutation
Asp 145	Gln 176	engineered mutation
Glu 153	Ala 184	engineered mutation
Gln 154	His 185	engineered mutation
Asn 155	Ala 186	engineered mutation
Ala 256	Pro 287	engineered mutation
Leu 305	Ile 336	engineered mutation
Phe 309	Thr 340	engineered mutation
Ile 313	Phe 344	engineered mutation

A, B, C, D, E: 393 - 419 (UniProt: P31644)

PDB	External Database	Details
Ser 316	-	linker
Gln 317	-	linker
Pro 318	-	linker
Ala 319	-	linker
Arg 320	-	linker
Ala 321	-	linker
Ala 322	-	linker
Ile 404	Val 435	engineered mutation
Gly 420	-	expression tag
Thr 421	-	expression tag
Thr 422	-	expression tag
Glu 423	-	expression tag
Thr 424	-	expression tag
Ser 425	-	expression tag
Gln 426	-	expression tag
Val 427	-	expression tag
Ala 428	-	expression tag
Pro 429	-	expression tag
Ala 430	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	205289.6
Non-Polymers*	Number of molecules	10
	Total formula weight	2866.7
All*	Total formula weight	208156.3

*Water molecules are not included.