8BH5

SARS-CoV-2 BA.2.12.1 RBD in complex with Beta-27 Fab and C1 nanobody

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (H)	Beta-27 heavy chain	polymer	222	23532.5	1		Homo sapiens
2	B (L)	Beta-27 light chain	polymer	214	23129.5	1		Homo sapiens
3	C (E)	Spike protein S1	polymer	202	23045.0	1	UniProt (P0DTC2) Pfam (PF09408)	Severe acute respiratory syndrome coronavirus 2
4	D (C)	Nanobody C1	polymer	131	14659.1	1		Lama glama
5	E (A)	alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		367.3	1	In PDB GlyTouCan (G86851RC)
6	F, G, H, I, K... (H, L, E, C)	GLYCEROL	non-polymer		92.1	12	Chemie (GOL)
7	J (H)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
8	S, T, U, V (H, L, E, C)	water	water		18.0	158	Chemie (HOH)

Sequence modifications

E: 333 - 528 (UniProt: P0DTC2)

PDB	External Database	Details
His 327	-	expression tag
His 328	-	expression tag
His 329	-	expression tag
His 330	-	expression tag
His 331	-	expression tag
His 332	-	expression tag
Asp 339	Gly 339	variant
Phe 371	Ser 371	variant
Pro 373	Ser 373	engineered mutation
Phe 375	Ser 375	variant
Ala 376	Thr 376	variant
Asn 405	Asp 405	variant
Ser 408	Arg 408	variant
Asn 417	Lys 417	variant
Lys 440	Asn 440	variant
Gln 452	Leu 452	variant
Asn 477	Ser 477	variant
Lys 478	Thr 478	variant
Ala 484	Glu 484	variant
Arg 493	Gln 493	variant
Arg 498	Gln 498	variant
Tyr 501	Asn 501	variant
His 505	Tyr 505	variant
Lys 527	Pro 527	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	84366.1
Branched	Number of molecules	1
	Total formula weight	367.3
Non-Polymers*	Number of molecules	13
	Total formula weight	1200.1
All*	Total formula weight	85933.6

*Water molecules are not included.