8BEU
Structure of D188A-fructofuranosidase from Rhodotorula dairenensis in complex with raffinose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Beta-fructofuranosidase | polymer | 675 | 70671.3 | 4 | UniProt (A0A856TAI5) Pfam (PF00251) Pfam (PF08244) | Rhodotorula dairenensis | |
| 2 | E, K (E, K) | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 2 | In PDB GlyTouCan (G81315DD) | |||
| 3 | F, I (F, I) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 2 | In PDB GlyTouCan (G42666HT) | |||
| 4 | G, J, L, N (G, J, L, N) | alpha-D-galactopyranose-(1-6)-alpha-D-glucopyranose-(1-2)-beta-D-fructofuranose | branched | 504.4 | 4 | In PDB BIRD (PRD_900002) | |||
| 5 | H (H) | alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G10756ZZ) | |||
| 6 | M (M) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 1 | In PDB GlyTouCan (G15407YE) | |||
| 7 | CA, EA, LA, MA, NA... (B, C, A, D) | alpha-D-mannopyranose | non-polymer | 180.2 | 13 | Chemie (MAN) | |||
| 8 | AA, AB, BA, BB, CB... (A, D, B, C) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 27 | Chemie (NAG) | |||
| 9 | DA (B) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 10 | DB, EB, FB, GB (A, B, C, D) | water | water | 18.0 | 556 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 675 (UniProt: A0A856TAI5)
| PDB | External Database | Details |
|---|---|---|
| Ala 188 | Asp 188 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 282685.1 | |
| Branched | Number of molecules | 10 |
| Total formula weight | 5861.3 | |
| Non-Polymers* | Number of molecules | 41 |
| Total formula weight | 8420.8 | |
| All* | Total formula weight | 296967.1 |
