8BES

Structure of D188A-fructofuranosidase from Rhodotorula dairenensis in complex with fructose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Beta-fructofuranosidase	polymer	675	70671.3	4	UniProt (A0A856TAI5) Pfam (PF00251) Pfam (PF08244) In PDB	Rhodotorula dairenensis
2	E	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G81315DD)
3	F, K	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	2	In PDB GlyTouCan (G15407YE)
4	G, I, J, L	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	4	In PDB GlyTouCan (G42666HT)
5	H	alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1073.0	1	In PDB GlyTouCan (G09724ZC)
6	B, D, C, A	alpha-D-mannopyranose	non-polymer		180.2	13	Chemie (MAN)
7	B, D, A, C	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	28	Chemie (NAG)
8	B, D, C, A	2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		209.2	4	Chemie (BTB)
9	B, D, C, A	beta-D-fructofuranose	non-polymer		180.2	4	Chemie (FRU)
10	D	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
11		water	water		18.0	1972	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 675 (UniProt: A0A856TAI5)

PDB	External Database	Details
Ala 188	Asp 188	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	282685.1
Branched	Number of molecules	8
	Total formula weight	4692.3
Non-Polymers*	Number of molecules	50
	Total formula weight	10199.6
All*	Total formula weight	297577.0

*Water molecules are not included.