8BEH
Cryo-EM structure of the Arabidopsis thaliana I+III2 supercomplex (CI membrane tip)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (L) | NADH-ubiquinone oxidoreductase chain 5 | polymer | 669 | 74498.0 | 1 | UniProt (P29388) Pfam (PF00662) Pfam (PF00361) Pfam (PF06455) | Arabidopsis thaliana (thale cress) | NADH dehydrogenase subunit 5 |
| 2 | B (M) | NADH-ubiquinone oxidoreductase chain 4 | polymer | 495 | 55995.7 | 1 | UniProt (P93313) Pfam (PF00361) | Arabidopsis thaliana (thale cress) | NADH dehydrogenase subunit 4 |
| 3 | C (T) | Acyl carrier protein 1, mitochondrial | polymer | 122 | 13735.6 | 1 | UniProt (P53665) Pfam (PF00550) | Arabidopsis thaliana (thale cress) | MtACP-1,ACP,NADH-ubiquinone oxidoreductase 9.6 kDa subunit |
| 4 | D (c) | Transmembrane protein | polymer | 88 | 9876.4 | 1 | UniProt (Q8VZT9) | Arabidopsis thaliana (thale cress) | |
| 5 | E (g) | ESSS subunit of NADH:ubiquinone oxidoreductase (Complex I) protein | polymer | 114 | 12648.3 | 1 | UniProt (Q9SLC8) | Arabidopsis thaliana (thale cress) | |
| 6 | F (i) | At1g67350 | polymer | 98 | 11808.2 | 1 | UniProt (Q9FYF8) Pfam (PF17250) | Arabidopsis thaliana (thale cress) | F1N21.17,NADH-ubiquinone oxidoreductase,P1 |
| 7 | G (j) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2 | polymer | 69 | 7582.6 | 1 | UniProt (Q8LDK3) | Arabidopsis thaliana (thale cress) | |
| 8 | H (k) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-A | polymer | 72 | 8064.3 | 1 | UniProt (O64725) Pfam (PF08122) | Arabidopsis thaliana (thale cress) | |
| 9 | I (l) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial | polymer | 125 | 13225.2 | 1 | UniProt (Q9FGK0) | Arabidopsis thaliana (thale cress) | |
| 10 | J (m) | AT2G31490 protein | polymer | 71 | 8305.5 | 1 | UniProt (Q9SIQ8) | Arabidopsis thaliana (thale cress) | At2g31490,Expressed protein,Neuronal acetylcholine receptor subunit alpha-5,Uncharacterized protein At2g31490,B15 -- 1 beta subcomplex subunit 4 |
| 11 | K (n) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 | polymer | 117 | 13638.3 | 1 | UniProt (Q945M1) | Arabidopsis thaliana (thale cress) | B22 subunit of eukaryotic mitochondrial complex I,Complex I-B22,CI-B22,NADH-ubiquinone oxidoreductase B22 subunit |
| 12 | L (o) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 | polymer | 103 | 11757.8 | 1 | UniProt (Q9SKC9) Pfam (PF05676) | Arabidopsis thaliana (thale cress) | |
| 13 | M (p) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10-B | polymer | 106 | 12462.3 | 1 | UniProt (Q94C12) Pfam (PF10249) | Arabidopsis thaliana (thale cress) | |
| 14 | N, R, T (L, M, l) | 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE | non-polymer | 705.0 | 3 | Chemie (3PH) | |||
| 15 | O (L) | (1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE | non-polymer | 751.0 | 1 | Chemie (PGT) | |||
| 16 | P (L) | PHOSPHATIDYLETHANOLAMINE | non-polymer | 734.0 | 1 | Chemie (PTY) | |||
| 17 | Q (L) | (7S)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE | non-polymer | 763.1 | 1 | Chemie (PC7) | |||
| 18 | S (T) | S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate | non-polymer | 540.7 | 1 | Chemie (8Q1) | |||
| 19 | AA, BA, CA, DA, EA... (j, k, l, m, n...) | water | water | 18.0 | 617 | Chemie (HOH) |
Sequence modifications
L: 1 - 669 (UniProt: P29388)
M: 1 - 495 (UniProt: P93313)
| PDB | External Database | Details |
|---|---|---|
| Phe 91 | Ser 91 | variant |
| Phe 288 | Ser 288 | variant |
| Leu 537 | Pro 537 | variant |
| PDB | External Database | Details |
|---|---|---|
| Phe 146 | Pro 146 | variant |
| Leu 326 | Pro 326 | variant |
| Phe 383 | Ser 383 | variant |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 13 |
| Total formula weight | 253598.3 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 4903.8 | |
| All* | Total formula weight | 258502.1 |
