8BE0

Early transcription elongation state of influenza B/Mem polymerase backtracked due to double incoproation of nucleotide analogue T1106 and with singly incoporated T1106 at the C +1 position

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Polymerase acidic protein	polymer	751	85822.8	1	UniProt (Q5V8Z9) Pfam (PF00603)	Influenza B virus (B/Memphis/13/2003)	RNA-directed RNA polymerase subunit P2
2	B (B)	RNA-directed RNA polymerase catalytic subunit	polymer	772	86207.0	1	UniProt (Q5V8Y6) Pfam (PF00602)	Influenza B virus (B/Memphis/13/2003)	Polymerase basic protein 1,PB1,RNA-directed RNA polymerase subunit P1
3	C (C)	Polymerase basic protein 2	polymer	798	90844.1	1	UniProt (Q5V8X3) Pfam (PF20947) Pfam (PF20948) Pfam (PF20949) Pfam (PF20950) Pfam (PF00604) Pfam (PF20951) Pfam (PF20952)	Influenza B virus (B/Memphis/13/2003)	RNA-directed RNA polymerase subunit P3
4	D (V)	5' vRNA	polymer	14	4495.7	1		Influenza B virus
5	E (R)	3' vRNA	polymer	21	6698.0	1		Influenza B virus
6	F (M)	mRNA	polymer	20	6308.8	1		Influenza B virus
7	G, H (B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
8	I (M)	P1-7-METHYLGUANOSINE-P3-ADENOSINE-5',5'-TRIPHOSPHATE	non-polymer		787.4	1	Chemie (GTA)
9	J, K, L, M, N... (A, B, C, V, R...)	water	water		18.0	95	Chemie (HOH)

Sequence modifications

A: 1 - 726 (UniProt: Q5V8Z9)

PDB	External Database	Details
Gly -13	-	expression tag
Ser -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gly 727	-	expression tag
Ser 728	-	expression tag
Gly 729	-	expression tag
Ser 730	-	expression tag
Gly 731	-	expression tag
Glu 732	-	expression tag
Asn 733	-	expression tag
Leu 734	-	expression tag
Tyr 735	-	expression tag
Phe 736	-	expression tag
Gln 737	-	expression tag

B: 1 - 752 (UniProt: Q5V8Y6)

PDB	External Database	Details
Gly -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Gly 753	-	expression tag
Ser 754	-	expression tag
Gly 755	-	expression tag
Ser 756	-	expression tag
Gly 757	-	expression tag
Glu 758	-	expression tag
Asn 759	-	expression tag
Leu 760	-	expression tag
Tyr 761	-	expression tag
Phe 762	-	expression tag
Gln 763	-	expression tag

C: 1 - 770 (UniProt: Q5V8X3)

PDB	External Database	Details
Gly -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Gly 771	-	expression tag
Trp 772	-	expression tag
Ser 773	-	expression tag
His 774	-	expression tag
Pro 775	-	expression tag
Gln 776	-	expression tag
Phe 777	-	expression tag
Glu 778	-	expression tag
Lys 779	-	expression tag
Gly 780	-	expression tag
Ser 781	-	expression tag
Gly 782	-	expression tag
Ser 783	-	expression tag
Glu 784	-	expression tag
Asn 785	-	expression tag
Leu 786	-	expression tag
Tyr 787	-	expression tag
Phe 788	-	expression tag
Gln 789	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	280376.4
Non-Polymers*	Number of molecules	3
	Total formula weight	836.1
All*	Total formula weight	281212.5

*Water molecules are not included.