8BD6

Cas12k-sgRNA-dsDNA-TnsC non-productive complex.

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Cas12k	polymer	698	79156.8	1	UniProt (A0A8M0FGU0) UniProt (by SIFTS) (A0A8X6EH11) In PDB	Scytonema hofmannii
2	B	sgRNA	polymer	256	82376.5	1		Scytonema hofmannii
3	C	DNA target strand	polymer	49	14916.6	1		synthetic construct
4	D	DNA non-target strand	polymer	49	15125.7	1		synthetic construct
5	R, S, T, U, V...	TnsC	polymer	276	31444.6	9	UniProt (A0A8J0PCL3) Pfam (PF05621) In PDB	Scytonema hofmannii
6	c, d	DNA	polymer	40	12303.0	2		synthetic construct
7	X, Y, Z, R, S...	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	9	Chemie (ATP)
8	W, X, Y, Z, R...	MAGNESIUM ION	non-polymer		24.3	9	Chemie (MG)

Sequence modifications

A: 2 - 639 (UniProt: A0A8M0FGU0)

PDB	External Database	Details
Met -58	-	initiating methionine
Gly -57	-	expression tag
Ser -56	-	expression tag
Ser -55	-	expression tag
His -54	-	expression tag
His -53	-	expression tag
His -52	-	expression tag
His -51	-	expression tag
His -50	-	expression tag
His -49	-	expression tag
Ser -48	-	expression tag
Gly -47	-	expression tag
Gly -46	-	expression tag
Gly -45	-	expression tag
Ser -44	-	expression tag
Gly -43	-	expression tag
Gly -42	-	expression tag
Ser -41	-	expression tag
Ala -40	-	expression tag
Trp -39	-	expression tag
Ser -38	-	expression tag
His -37	-	expression tag
Pro -36	-	expression tag
Gln -35	-	expression tag
Phe -34	-	expression tag
Glu -33	-	expression tag
Lys -32	-	expression tag
Gly -31	-	expression tag
Gly -30	-	expression tag
Gly -29	-	expression tag
Ser -28	-	expression tag
Gly -27	-	expression tag
Gly -26	-	expression tag
Gly -25	-	expression tag
Ser -24	-	expression tag
Gly -23	-	expression tag
Gly -22	-	expression tag
Ser -21	-	expression tag
Ala -20	-	expression tag
Trp -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
Pro -16	-	expression tag
Gln -15	-	expression tag
Phe -14	-	expression tag
Glu -13	-	expression tag
Lys -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Gly -9	-	expression tag
Gly -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Ser -1	-	expression tag
Asn 0	-	expression tag
Ala 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	15
	Total formula weight	499183.3
Non-Polymers*	Number of molecules	18
	Total formula weight	4783.4
All*	Total formula weight	503966.7

*Water molecules are not included.