8BD5
Cas12k-sgRNA-dsDNA-S15-TniQ-TnsC transposon recruitment complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | ShCas12k | polymer | 698 | 79156.8 | 1 | UniProt (A0A8X6EH11) | Scytonema hofmannii | |
| 2 | B (B) | sgRNA | polymer | 256 | 82376.5 | 1 | Scytonema hofmannii | ||
| 3 | C (C) | DNA target strand | polymer | 49 | 14916.6 | 1 | synthetic construct | ||
| 4 | D (D) | DNA non-target strand | polymer | 49 | 15125.7 | 1 | synthetic construct | ||
| 5 | E, F, G, H, I... (E, F, G, H, I...) | TnsC | polymer | 276 | 31444.6 | 7 | UniProt (A0A8J0PCL3) Pfam (PF05621) | Scytonema hofmannii | |
| 6 | L (Q) | TniQ (Homology model) | polymer | 167 | 19011.2 | 1 | UniProt (A0A8J0PCL5) Pfam (PF06527) | Scytonema hofmannii | |
| 7 | M (X) | 30S ribosomal protein S15 | polymer | 89 | 10290.8 | 1 | UniProt (P0ADZ4) Pfam (PF00312) | Escherichia coli K-12 | Small ribosomal subunit protein uS15 |
| 8 | N, P, R, T, V... (E, F, G, H, I...) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 7 | Chemie (ATP) | |||
| 9 | AA, O, Q, S, U... (K, E, F, G, H...) | MAGNESIUM ION | non-polymer | 24.3 | 7 | Chemie (MG) | |||
| 10 | BA, CA (Q) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
A: -1 - 639 (UniProt: A0A8X6EH11)
| PDB | External Database | Details |
|---|---|---|
| Met -58 | - | initiating methionine |
| Gly -57 | - | expression tag |
| Ser -56 | - | expression tag |
| Ser -55 | - | expression tag |
| His -54 | - | expression tag |
| His -53 | - | expression tag |
| His -52 | - | expression tag |
| His -51 | - | expression tag |
| His -50 | - | expression tag |
| His -49 | - | expression tag |
| Ser -48 | - | expression tag |
| Gly -47 | - | expression tag |
| Gly -46 | - | expression tag |
| Gly -45 | - | expression tag |
| Ser -44 | - | expression tag |
| Gly -43 | - | expression tag |
| Gly -42 | - | expression tag |
| Ser -41 | - | expression tag |
| Ala -40 | - | expression tag |
| Trp -39 | - | expression tag |
| Ser -38 | - | expression tag |
| His -37 | - | expression tag |
| Pro -36 | - | expression tag |
| Gln -35 | - | expression tag |
| Phe -34 | - | expression tag |
| Glu -33 | - | expression tag |
| Lys -32 | - | expression tag |
| Gly -31 | - | expression tag |
| Gly -30 | - | expression tag |
| Gly -29 | - | expression tag |
| Ser -28 | - | expression tag |
| Gly -27 | - | expression tag |
| Gly -26 | - | expression tag |
| Gly -25 | - | expression tag |
| Ser -24 | - | expression tag |
| Gly -23 | - | expression tag |
| Gly -22 | - | expression tag |
| Ser -21 | - | expression tag |
| Ala -20 | - | expression tag |
| Trp -19 | - | expression tag |
| Ser -18 | - | expression tag |
| His -17 | - | expression tag |
| Pro -16 | - | expression tag |
| Gln -15 | - | expression tag |
| Phe -14 | - | expression tag |
| Glu -13 | - | expression tag |
| Lys -12 | - | expression tag |
| Ser -11 | - | expression tag |
| Gly -10 | - | expression tag |
| Gly -9 | - | expression tag |
| Gly -8 | - | expression tag |
| Glu -7 | - | expression tag |
| Asn -6 | - | expression tag |
| Leu -5 | - | expression tag |
| Tyr -4 | - | expression tag |
| Phe -3 | - | expression tag |
| Gln -2 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 13 |
| Total formula weight | 440990.0 | |
| Non-Polymers* | Number of molecules | 16 |
| Total formula weight | 3851.2 | |
| All* | Total formula weight | 444841.3 |
