8BBI
Crystal structure of Xyn11 double mutant L271S, K275H from Psedothermotoga thermarum
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Beta-xylanase | polymer | 368 | 43212.1 | 2 | UniProt (F7YXD6) Pfam (PF00331) In PDB | Pseudothermotoga thermarum DSM 5069 | |
2 | A, B | GLYCEROL | non-polymer | 92.1 | 6 | Chemie (GOL) | |||
3 | A, B | 1-methylethyl 1-thio-beta-D-galactopyranoside | non-polymer | 238.3 | 2 | Chemie (IPT) | |||
4 | water | water | 18.0 | 275 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 343 (UniProt: F7YXD6)
PDB | External Database | Details |
---|---|---|
Met -15 | - | initiating methionine |
Arg -14 | - | expression tag |
Gly -13 | - | expression tag |
Ser -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
His -9 | - | expression tag |
His -8 | - | expression tag |
His -7 | - | expression tag |
His -6 | - | expression tag |
Gly -5 | - | expression tag |
Ser -4 | - | expression tag |
Ala -3 | - | expression tag |
Cys -2 | - | expression tag |
Glu -1 | - | expression tag |
Leu 0 | - | expression tag |
Ser 271 | Leu 292 | engineered mutation |
His 275 | Lys 296 | engineered mutation |
Val 344 | - | expression tag |
Asp 345 | - | expression tag |
Leu 346 | - | expression tag |
Gln 347 | - | expression tag |
Pro 348 | - | expression tag |
Ser 349 | - | expression tag |
Leu 350 | - | expression tag |
Ile 351 | - | expression tag |
Ser 352 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 86424.3 | |
Non-Polymers* | Number of molecules | 8 |
Total formula weight | 1029.2 | |
All* | Total formula weight | 87453.4 |