8BBI

Crystal structure of Xyn11 double mutant L271S, K275H from Psedothermotoga thermarum

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Beta-xylanase	polymer	368	43212.1	2	UniProt (F7YXD6) Pfam (PF00331)	Pseudothermotoga thermarum DSM 5069
2	C, D, E, G, H... (A, B)	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
3	F, I (A, B)	1-methylethyl 1-thio-beta-D-galactopyranoside	non-polymer		238.3	2	Chemie (IPT)
4	K, L (A, B)	water	water		18.0	275	Chemie (HOH)

Sequence modifications

A, B: 1 - 343 (UniProt: F7YXD6)

PDB	External Database	Details
Met -15	-	initiating methionine
Arg -14	-	expression tag
Gly -13	-	expression tag
Ser -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
Ala -3	-	expression tag
Cys -2	-	expression tag
Glu -1	-	expression tag
Leu 0	-	expression tag
Ser 271	Leu 292	engineered mutation
His 275	Lys 296	engineered mutation
Val 344	-	expression tag
Asp 345	-	expression tag
Leu 346	-	expression tag
Gln 347	-	expression tag
Pro 348	-	expression tag
Ser 349	-	expression tag
Leu 350	-	expression tag
Ile 351	-	expression tag
Ser 352	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	86424.3
Non-Polymers*	Number of molecules	8
	Total formula weight	1029.2
All*	Total formula weight	87453.4

*Water molecules are not included.