8BBI
Crystal structure of Xyn11 double mutant L271S, K275H from Psedothermotoga thermarum
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Beta-xylanase | polymer | 368 | 43212.1 | 2 | UniProt (F7YXD6) Pfam (PF00331) | Pseudothermotoga thermarum DSM 5069 | |
| 2 | C, D, E, G, H... (A, B) | GLYCEROL | non-polymer | 92.1 | 6 | Chemie (GOL) PubChem (753) | |||
| 3 | F, I (A, B) | 1-methylethyl 1-thio-beta-D-galactopyranoside | non-polymer | 238.3 | 2 | Chemie (IPT) PubChem (16760528) PubChem (2802070) PubChem (9711) PubChem (22819629) PubChem (22836579) PubChem (16217509) PubChem (24802248) PubChem (25113457) PubChem (25322847) PubChem (26470596) PubChem (26470897) PubChem (26470899) PubChem (38989032) PubChem (40489056) PubChem (552632) PubChem (656894) PubChem (853436) PubChem (53694108) PubChem (69133132) PubChem (70877296) PubChem (6928325) PubChem (92223493) PubChem (92843742) PubChem (92843743) PubChem (95387455) PubChem (95387456) PubChem (134694195) PubChem (122599329) PubChem (129353964) PubChem (131676754) PubChem (140882545) PubChem (140882549) PubChem (172897862) PubChem (175675668) | |||
| 4 | K, L (A, B) | water | water | 18.0 | 275 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 343 (UniProt: F7YXD6)
| PDB | External Database | Details |
|---|---|---|
| Met -15 | - | initiating methionine |
| Arg -14 | - | expression tag |
| Gly -13 | - | expression tag |
| Ser -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| Gly -5 | - | expression tag |
| Ser -4 | - | expression tag |
| Ala -3 | - | expression tag |
| Cys -2 | - | expression tag |
| Glu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Ser 271 | Leu 292 | engineered mutation |
| His 275 | Lys 296 | engineered mutation |
| Val 344 | - | expression tag |
| Asp 345 | - | expression tag |
| Leu 346 | - | expression tag |
| Gln 347 | - | expression tag |
| Pro 348 | - | expression tag |
| Ser 349 | - | expression tag |
| Leu 350 | - | expression tag |
| Ile 351 | - | expression tag |
| Ser 352 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 86424.3 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 1029.2 | |
| All* | Total formula weight | 87453.4 |
