8B9B

S. cerevisiae replisome + Ctf4, bound by pol alpha. Complex engaged with a fork DNA substrate containing a 60 nucleotide lagging strand.

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	2	DNA replication licensing factor MCM2	polymer	868	98911.5	1	UniProt (P29469) Pfam (PF12619) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) In PDB	Saccharomyces cerevisiae (baker's yeast)	Minichromosome maintenance protein 2
2	3	DNA replication licensing factor MCM3	polymer	1009	111987.6	1	UniProt (P24279) Pfam (PF22207) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) In PDB	Saccharomyces cerevisiae (baker's yeast)	Minichromosome maintenance protein 3
3	4	DNA replication licensing factor MCM4	polymer	933	105138.4	1	UniProt (P30665) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21128) In PDB	Saccharomyces cerevisiae (baker's yeast)	Cell division control protein 54
4	5	Minichromosome maintenance protein 5	polymer	775	86505.7	1	UniProt (P29496) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21933) In PDB	Saccharomyces cerevisiae (baker's yeast)	Cell division control protein 46
5	6	DNA replication licensing factor MCM6	polymer	1017	113110.2	1	UniProt (P53091) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF18263) In PDB	Saccharomyces cerevisiae (baker's yeast)	Minichromosome maintenance protein 6
6	7	DNA replication licensing factor MCM7	polymer	845	95049.9	1	UniProt (P38132) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) In PDB	Saccharomyces cerevisiae (baker's yeast)	Cell division control protein 47,Minichromosome maintenance protein 7
7	A	DNA primase large subunit	polymer	528	62348.6	1	UniProt (P20457) Pfam (PF04104) In PDB	Saccharomyces cerevisiae (baker's yeast)	DNA polymerase alpha:primase complex p58 subunit,DNA polymerase-primase complex p58 subunit,Pol alpha-primase complex p58 subunit,DNA primase 58 kDa subunit
8	B	DNA polymerase alpha subunit B	polymer	705	78865.9	1	UniProt (P38121) Pfam (PF22062) Pfam (PF04042) In PDB	Saccharomyces cerevisiae (baker's yeast)	DNA polymerase I subunit B,DNA polymerase alpha:primase complex p86 subunit,Pol alpha-primase complex p86 subunit,DNA polymerase-primase complex p74 subunit
9	C	DNA replication complex GINS protein PSF1	polymer	208	24230.6	1	UniProt (Q12488) Pfam (PF05916) In PDB	Saccharomyces cerevisiae (baker's yeast)	Partner of Sld five 1
10	D	DNA replication complex GINS protein PSF2	polymer	213	25096.8	1	UniProt (P40359) Pfam (PF05916) In PDB	Saccharomyces cerevisiae (baker's yeast)	Partner of Sld five 2
11	E	DNA replication complex GINS protein PSF3	polymer	217	24437.9	1	UniProt (Q12146) Pfam (PF22466) Pfam (PF05916) In PDB	Saccharomyces cerevisiae (baker's yeast)	Partner of Sld five 3
12	F	DNA replication complex GINS protein SLD5	polymer	294	33983.6	1	UniProt (Q03406) Pfam (PF05916) Pfam (PF16922) In PDB	Saccharomyces cerevisiae (baker's yeast)
13	G	Cell division control protein 45	polymer	657	75154.7	1	UniProt (Q08032) Pfam (PF02724) In PDB	Saccharomyces cerevisiae (baker's yeast)
14	J	DNA polymerase alpha catalytic subunit A	polymer	1468	167027.8	1	UniProt (P13382) Pfam (PF12254) Pfam (PF03104) Pfam (PF00136) Pfam (PF08996) In PDB	Saccharomyces cerevisiae (baker's yeast)	DNA polymerase I subunit A,DNA polymerase alpha:primase complex p180 subunit,DNA polymerase-primase complex p180 subunit,Pol alpha-primase complex p180 subunit
15	P	Mediator of replication checkpoint protein 1	polymer	1109	126078.9	1	UniProt (P25588) Pfam (PF09444) In PDB	Saccharomyces cerevisiae (baker's yeast)	DNA replication checkpoint mediator MRC1
16	Q	Leading strand DNA	polymer	84	26092.6	1		Saccharomyces cerevisiae
17	R	Lagging strand DNA	polymer	106	32046.5	1		Saccharomyces cerevisiae
18	S	DNA primase small subunit	polymer	444	51827.1	1	UniProt (P10363) Pfam (PF01896) In PDB	Saccharomyces cerevisiae (baker's yeast)	DNA polymerase alpha:primase complex p48 subunit,DNA polymerase-primase complex p48 subunit,Pol alpha-primase complex p48 subunit,DNA primase 48 kDa subunit
19	X	Topoisomerase 1-associated factor 1	polymer	1238	141296.9	1	UniProt (P53840) Pfam (PF04821) In PDB	Saccharomyces cerevisiae (baker's yeast)
20	Y	Chromosome segregation in meiosis protein 3	polymer	319	36588.8	1	UniProt (Q04659) Pfam (PF07962) In PDB	Saccharomyces cerevisiae (baker's yeast)
21	H, L, K	DNA polymerase alpha-binding protein	polymer	962	108610.1	3	UniProt (Q01454) Pfam (PF12341) Pfam (PF20946) In PDB	Saccharomyces cerevisiae (baker's yeast)	Chromosome replication protein CHL15,Chromosome transmission fidelity protein 4,Protein POB1
22	4, 7, 2, 3	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	4	Chemie (ANP)
23	4, 5, 7, 2	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
24	4, 5, 6, 7	ZINC ION	non-polymer		65.4	4	Chemie (ZN)

Sequence modifications

3: 1 - 971 (UniProt: P24279)

PDB	External Database	Details
Met -37	-	initiating methionine
Lys -36	-	expression tag
Arg -35	-	expression tag
Arg -34	-	expression tag
Trp -33	-	expression tag
Lys -32	-	expression tag
Lys -31	-	expression tag
Asn -30	-	expression tag
Phe -29	-	expression tag
Ile -28	-	expression tag
Ala -27	-	expression tag
Val -26	-	expression tag
Ser -25	-	expression tag
Ala -24	-	expression tag
Ala -23	-	expression tag
Asn -22	-	expression tag
Arg -21	-	expression tag
Phe -20	-	expression tag
Lys -19	-	expression tag
Lys -18	-	expression tag
Ile -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
Ser -14	-	expression tag
Gly -13	-	expression tag
Ala -12	-	expression tag
Leu -11	-	expression tag
Glu -10	-	expression tag
Asn -9	-	expression tag
Leu -8	-	expression tag
Tyr -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Glu -3	-	expression tag
Ala -2	-	expression tag
Pro -1	-	expression tag
Val 0	-	expression tag

E: 1 - 194 (UniProt: Q12146)

PDB	External Database	Details
Met -22	-	initiating methionine
Gly -21	-	expression tag
Ser -20	-	expression tag
Ser -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Leu -9	-	expression tag
Val -8	-	expression tag
Pro -7	-	expression tag
Arg -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag

G: 1 - 167 (UniProt: Q08032)
G: 167 - 650 (UniProt: Q08032)

PDB	External Database	Details
Tyr 167	-	linker
Lys 167	-	linker
Asp 167	-	linker
Asp 167	-	linker
Asp 167	-	linker
Gly 167	-	linker
Asp 167	-	linker
Tyr 167	-	linker
Lys 167	-	linker
Asp 167	-	linker
Asp 167	-	linker

P: 1 - 1096 (UniProt: P25588)

PDB	External Database	Details
Asp 1097	-	expression tag
Tyr 1098	-	expression tag
Lys 1099	-	expression tag
Asp 1100	-	expression tag
Asp 1101	-	expression tag
Asp 1102	-	expression tag
Gly 1103	-	expression tag
Asp 1104	-	expression tag
Tyr 1105	-	expression tag
Lys 1106	-	expression tag
Asp 1107	-	expression tag
Asp 1108	-	expression tag
Asp 1109	-	expression tag

S: 1 - 409 (UniProt: P10363)

PDB	External Database	Details
Met -34	-	initiating methionine
Lys -33	-	expression tag
Arg -32	-	expression tag
Arg -31	-	expression tag
Trp -30	-	expression tag
Lys -29	-	expression tag
Lys -28	-	expression tag
Asn -27	-	expression tag
Phe -26	-	expression tag
Ile -25	-	expression tag
Ala -24	-	expression tag
Val -23	-	expression tag
Ser -22	-	expression tag
Ala -21	-	expression tag
Ala -20	-	expression tag
Asn -19	-	expression tag
Arg -18	-	expression tag
Phe -17	-	expression tag
Lys -16	-	expression tag
Lys -15	-	expression tag
Ile -14	-	expression tag
Ser -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Ala -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Glu 0	-	expression tag

Y: 1 - 317 (UniProt: Q04659)

PDB	External Database	Details
Gly -1	-	expression tag
Glu 0	-	expression tag

H, L, K: 1 - 927 (UniProt: Q01454)

PDB	External Database	Details
Met -34	-	initiating methionine
Lys -33	-	expression tag
Arg -32	-	expression tag
Arg -31	-	expression tag
Trp -30	-	expression tag
Lys -29	-	expression tag
Lys -28	-	expression tag
Asn -27	-	expression tag
Phe -26	-	expression tag
Ile -25	-	expression tag
Ala -24	-	expression tag
Val -23	-	expression tag
Ser -22	-	expression tag
Ala -21	-	expression tag
Ala -20	-	expression tag
Asn -19	-	expression tag
Arg -18	-	expression tag
Phe -17	-	expression tag
Lys -16	-	expression tag
Lys -15	-	expression tag
Ile -14	-	expression tag
Ser -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Ala -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Glu 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	23
	Total formula weight	1841610.2
Non-Polymers*	Number of molecules	12
	Total formula weight	2383.6
All*	Total formula weight	1843993.9

*Water molecules are not included.