8B77
The crystal structure of N828V variant of DNA Pol Epsilon containing dATP in the polymerase active site
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase epsilon catalytic subunit A | polymer | 1191 | 137058.6 | 1 | UniProt (P21951) Pfam (PF03104) Pfam (PF22634) | Saccharomyces cerevisiae (baker's yeast) | 3'-5' exodeoxyribonuclease,DNA polymerase II subunit A |
| 2 | B (P) | Primer DNA sequence | polymer | 11 | 3293.2 | 1 | synthetic construct | ||
| 3 | C (T) | Template DNA sequence | polymer | 16 | 4889.2 | 1 | synthetic construct | ||
| 4 | D (A) | 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE | non-polymer | 491.2 | 1 | Chemie (DTP) | |||
| 5 | E (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) |
Sequence modifications
A: 1 - 1186 (UniProt: P21951)
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Pro -1 | - | expression tag |
| His 0 | - | expression tag |
| Ala 290 | Asp 290 | engineered mutation |
| Ala 292 | Glu 292 | engineered mutation |
| Val 828 | Asn 828 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 145240.9 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 531.3 | |
| All* | Total formula weight | 145772.2 |
