8B6O
X-ray structure of the haloalkane dehalogenase HaloTag7 circular permutated at positions 141-156 (cpHaloTagDelta) fused to M13
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Haloalkane dehalogenase | polymer | 323 | 35720.7 | 1 | UniProt (P0A3G3) Pfam (PF00561) | Rhodococcus sp. | |
| 2 | B (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 3 | C (A) | water | water | 18.0 | 47 | Chemie (HOH) |
Sequence modifications
A: 31 - 168 (UniProt: P0A3G3)
A: 186 - 323 (UniProt: P0A3G3)
| PDB | External Database | Details |
|---|---|---|
| Gly 1 | - | expression tag |
| Met 2 | - | expression tag |
| Val 3 | - | expression tag |
| Asp 4 | - | expression tag |
| Ser 5 | - | expression tag |
| Ser 6 | - | expression tag |
| Arg 7 | - | expression tag |
| Arg 8 | - | expression tag |
| Lys 9 | - | expression tag |
| Trp 10 | - | expression tag |
| Asn 11 | - | expression tag |
| Lys 12 | - | expression tag |
| Thr 13 | - | expression tag |
| Gly 14 | - | expression tag |
| His 15 | - | expression tag |
| Ala 16 | - | expression tag |
| Val 17 | - | expression tag |
| Arg 18 | - | expression tag |
| Ala 19 | - | expression tag |
| Ile 20 | - | expression tag |
| Gly 21 | - | expression tag |
| Arg 22 | - | expression tag |
| Leu 23 | - | expression tag |
| Ser 24 | - | expression tag |
| Ser 25 | - | expression tag |
| Leu 26 | - | expression tag |
| Glu 27 | - | expression tag |
| Gly 28 | - | expression tag |
| Gly 29 | - | expression tag |
| Ser 30 | - | expression tag |
| Lys 35 | Glu 160 | conflict |
| Val 42 | Ala 167 | conflict |
| Thr 47 | Ala 172 | conflict |
| Met 50 | Lys 175 | conflict |
| Gly 51 | Cys 176 | conflict |
| Asn 70 | Lys 195 | conflict |
| Glu 99 | Ala 224 | conflict |
| Asp 102 | Asn 227 | conflict |
| Lys 132 | Glu 257 | conflict |
| Ala 139 | Thr 264 | conflict |
| Asn 147 | His 272 | conflict |
| Leu 148 | Tyr 273 | conflict |
| Ser 166 | Pro 291 | conflict |
| Thr 167 | Ala 292 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Glu 169 | - | linker |
| Ile 170 | - | linker |
| Gly 171 | - | linker |
| Gly 172 | - | linker |
| Thr 173 | - | linker |
| Gly 174 | - | linker |
| Gly 175 | - | linker |
| Ser 176 | - | linker |
| Gly 177 | - | linker |
| Gly 178 | - | linker |
| Thr 179 | - | linker |
| Gly 180 | - | linker |
| Gly 181 | - | linker |
| Ser 182 | - | linker |
| Gly 183 | - | linker |
| Gly 184 | - | linker |
| Ser 185 | - | linker |
| Val 229 | Leu 47 | conflict |
| Thr 240 | Ser 58 | conflict |
| Gly 260 | Asp 78 | conflict |
| Phe 269 | Tyr 87 | conflict |
| Met 270 | Leu 88 | conflict |
| Phe 310 | Cys 128 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 35720.7 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 35.5 | |
| All* | Total formula weight | 35756.1 |
