8B6N
X-ray structure of the haloalkane dehalogenase HaloTag7 circular permutated at positions 141-156 (cpHaloTagDelta)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Haloalkane dehalogenase | polymer | 294 | 32575.1 | 1 | UniProt (P0A3G3) | Rhodococcus sp. | |
| 2 | B (A) | 2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID | non-polymer | 207.3 | 1 | Chemie (NHE) | |||
| 3 | C (A) | water | water | 18.0 | 13 | Chemie (HOH) |
Sequence modifications
A: 2 - 139 (UniProt: P0A3G3)
A: 157 - 294 (UniProt: P0A3G3)
| PDB | External Database | Details |
|---|---|---|
| Gly 1 | - | expression tag |
| Lys 6 | Glu 160 | conflict |
| Val 13 | Ala 167 | conflict |
| Thr 18 | Ala 172 | conflict |
| Met 21 | Lys 175 | conflict |
| Gly 22 | Cys 176 | conflict |
| Asn 41 | Lys 195 | conflict |
| Glu 70 | Ala 224 | conflict |
| Asp 73 | Asn 227 | conflict |
| Lys 103 | Glu 257 | conflict |
| Ala 110 | Thr 264 | conflict |
| Asn 118 | His 272 | conflict |
| Leu 119 | Tyr 273 | conflict |
| Ser 137 | Pro 291 | conflict |
| Thr 138 | Ala 292 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Glu 140 | - | linker |
| Ile 141 | - | linker |
| Gly 142 | - | linker |
| Gly 143 | - | linker |
| Thr 144 | - | linker |
| Gly 145 | - | linker |
| Gly 146 | - | linker |
| Ser 147 | - | linker |
| Gly 148 | - | linker |
| Gly 149 | - | linker |
| Thr 150 | - | linker |
| Gly 151 | - | linker |
| Gly 152 | - | linker |
| Ser 153 | - | linker |
| Gly 154 | - | linker |
| Gly 155 | - | linker |
| Ser 156 | - | linker |
| Val 200 | Leu 47 | conflict |
| Thr 211 | Ser 58 | conflict |
| Gly 231 | Asp 78 | conflict |
| Phe 240 | Tyr 87 | conflict |
| Met 241 | Leu 88 | conflict |
| Phe 281 | Cys 128 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 32575.1 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 207.3 | |
| All* | Total formula weight | 32782.4 |
