8B67
The crystal structure of M644G variant of DNA Pol Epsilon containing CTP in the polymerase active site
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase epsilon catalytic subunit A | polymer | 1191 | 137101.5 | 1 | UniProt (P21951) Pfam (PF03104) Pfam (PF22634) | Saccharomyces cerevisiae (baker's yeast) | 3'-5' exodeoxyribonuclease,DNA polymerase II subunit A |
| 2 | B (P) | Primer DNA sequence | polymer | 11 | 3293.2 | 1 | synthetic construct | ||
| 3 | C (T) | Template DNA sequence | polymer | 16 | 4914.2 | 1 | synthetic construct | ||
| 4 | D, E, F (A) | CALCIUM ION | non-polymer | 40.1 | 3 | Chemie (CA) | |||
| 5 | G (A) | CYTIDINE-5'-TRIPHOSPHATE | non-polymer | 483.2 | 1 | Chemie (CTP) | |||
| 6 | H (A) | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) | |||
| 7 | I, J, K (A, P, T) | water | water | 18.0 | 15 | Chemie (HOH) |
Sequence modifications
A: 1 - 1186 (UniProt: P21951)
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Pro -1 | - | expression tag |
| His 0 | - | expression tag |
| Gly 644 | Met 644 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 145308.8 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 662.4 | |
| All* | Total formula weight | 145971.3 |
