8B65

Structure of rsCherry crystallized in anaerobic conditions

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	rsCherry	polymer	271	30709.4	1	UniProt (Q9U6Y8) Pfam (PF01353) UniProt (by SIFTS) (D1MPT3) In PDB	Discosoma sp.
2	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
3	A	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
4	A	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
5	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6		water	water		18.0	269	Chemie (HOH)

Sequence modifications

A: 8 - 221 (UniProt: Q9U6Y8)

PDB	External Database	Details
Met -39	-	initiating methionine
Arg -38	-	expression tag
Gly -37	-	expression tag
Ser -36	-	expression tag
His -35	-	expression tag
His -34	-	expression tag
His -33	-	expression tag
His -32	-	expression tag
His -31	-	expression tag
His -30	-	expression tag
Gly -29	-	expression tag
Met -28	-	expression tag
Ala -27	-	expression tag
Ser -26	-	expression tag
Met -25	-	expression tag
Thr -24	-	expression tag
Gly -23	-	expression tag
Gly -22	-	expression tag
Gln -21	-	expression tag
Gln -20	-	expression tag
Met -19	-	expression tag
Gly -18	-	expression tag
Arg -17	-	expression tag
Asp -16	-	expression tag
Leu -15	-	expression tag
Tyr -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Asp -11	-	expression tag
Asp -10	-	expression tag
Lys -9	-	expression tag
Asp -8	-	expression tag
Pro -7	-	expression tag
Ala -6	-	expression tag
Thr -5	-	expression tag
Met -4	-	expression tag
Val -3	-	expression tag
Ser -2	-	expression tag
Lys -1	-	expression tag
Gly 0	-	expression tag
Glu 1	-	expression tag
Glu 2	-	expression tag
Asp 3	-	expression tag
Asn 4	-	expression tag
Met 5	-	expression tag
Ala 6	-	expression tag
Ile 7	-	expression tag
His 17	Arg 17	engineered mutation
Ser 21	Thr 21	engineered mutation
Thr 41	His 41	engineered mutation
Gln 42	Asn 42	engineered mutation
Ala 44	Val 44	engineered mutation
Qyx 66	Gln 66	chromophore
Qyx 66	Tyr 67	chromophore
Qyx 66	Gly 68	chromophore
Ala 71	Val 71	engineered mutation
Leu 83	Lys 83	engineered mutation
Glu 117	Cys 117	engineered mutation
Leu 124	Phe 124	engineered mutation
Arg 125	Ile 125	engineered mutation
Thr 127	Val 127	engineered mutation
Val 144	Glu 144	engineered mutation
Ser 147	Thr 147	engineered mutation
Met 150	Leu 150	engineered mutation
Glu 153	Arg 153	engineered mutation
Ala 156	Val 156	engineered mutation
Ser 161	Ile 161	engineered mutation
Lys 162	His 162	engineered mutation
Gln 163	Lys 163	engineered mutation
Arg 164	Ala 164	engineered mutation
Asp 174	Leu 174	engineered mutation
Ala 175	Val 175	engineered mutation
Trp 178	Lys 178	engineered mutation
Thr 179	Ser 179	engineered mutation
Thr 180	Ile 180	engineered mutation
Lys 182	Met 182	engineered mutation
Ala 192	Tyr 192	engineered mutation
Asn 194	Tyr 194	engineered mutation
Asn 196	Asp 196	engineered mutation
Ile 197	Ser 197	engineered mutation
Ala 217	Thr 217	engineered mutation
Ser 222	-	expression tag
Thr 223	-	expression tag
Gly 224	-	expression tag
Gly 225	-	expression tag
Met 226	-	expression tag
Asp 227	-	expression tag
Glu 228	-	expression tag
Leu 229	-	expression tag
Tyr 230	-	expression tag
Lys 231	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	30709.4
Non-Polymers*	Number of molecules	5
	Total formula weight	517.6
All*	Total formula weight	31227.0

*Water molecules are not included.