8B2D
CRYSTAL STRUCTURE OF BACTERIAL FLAVIN CONTAINING MONOOXYGENASE THERMORESISTANT MUTANT, IN COMPLEX WITH NADP+
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Flavin-containing monooxygenase, Fmo | polymer | 464 | 54131.3 | 2 | UniProt (F5SYD3) Pfam (PF00743) | Methylophaga aminisulfidivorans MP | |
| 2 | C, K (A, B) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 3 | D, E, H, J, N... (A, B) | GLYCEROL | non-polymer | 92.1 | 6 | Chemie (GOL) | |||
| 4 | F, L (A, B) | FLAVIN-ADENINE DINUCLEOTIDE | non-polymer | 785.5 | 2 | Chemie (FAD) | |||
| 5 | G, M (A, B) | NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 743.4 | 2 | Chemie (NAP) | |||
| 6 | I (B) | 2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 209.2 | 1 | Chemie (BTB) | |||
| 7 | P, Q (B) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 8 | R, S (A, B) | water | water | 18.0 | 495 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 456 (UniProt: F5SYD3)
| PDB | External Database | Details |
|---|---|---|
| Pro 95 | Ala 95 | engineered mutation |
| Thr 135 | Ser 135 | engineered mutation |
| Gly 197 | Asp 197 | engineered mutation |
| Val 224 | Leu 224 | engineered mutation |
| Asp 236 | Lys 236 | engineered mutation |
| Gly 252 | Thr 252 | engineered mutation |
| Asp 290 | Asn 290 | engineered mutation |
| Pro 296 | Leu 296 | engineered mutation |
| Ile 340 | Leu 340 | engineered mutation |
| Gln 353 | Met 353 | engineered mutation |
| Arg 358 | Lys 358 | engineered mutation |
| Glu 360 | Leu 360 | engineered mutation |
| Lys 363 | Val 363 | engineered mutation |
| Asp 365 | Ala 365 | engineered mutation |
| Asp 370 | Thr 370 | engineered mutation |
| Phe 371 | Tyr 371 | engineered mutation |
| Asp 378 | Asn 378 | engineered mutation |
| Val 393 | Thr 393 | engineered mutation |
| Lys 398 | Leu 398 | engineered mutation |
| Phe 443 | Tyr 443 | engineered mutation |
| Leu 457 | - | expression tag |
| Glu 458 | - | expression tag |
| His 459 | - | expression tag |
| His 460 | - | expression tag |
| His 461 | - | expression tag |
| His 462 | - | expression tag |
| His 463 | - | expression tag |
| His 464 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 108262.6 | |
| Non-Polymers* | Number of molecules | 15 |
| Total formula weight | 4082.7 | |
| All* | Total formula weight | 112345.3 |
