8B2D
CRYSTAL STRUCTURE OF BACTERIAL FLAVIN CONTAINING MONOOXYGENASE THERMORESISTANT MUTANT, IN COMPLEX WITH NADP+
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Flavin-containing monooxygenase, Fmo | polymer | 464 | 54131.3 | 2 | UniProt (F5SYD3) Pfam (PF00743) In PDB | Methylophaga aminisulfidivorans MP | |
2 | A, B | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
3 | A, B | GLYCEROL | non-polymer | 92.1 | 6 | Chemie (GOL) | |||
4 | A, B | FLAVIN-ADENINE DINUCLEOTIDE | non-polymer | 785.5 | 2 | Chemie (FAD) | |||
5 | A, B | NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 743.4 | 2 | Chemie (NAP) | |||
6 | B | 2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 209.2 | 1 | Chemie (BTB) | |||
7 | B | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
8 | water | water | 18.0 | 495 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 456 (UniProt: F5SYD3)
PDB | External Database | Details |
---|---|---|
Pro 95 | Ala 95 | engineered mutation |
Thr 135 | Ser 135 | engineered mutation |
Gly 197 | Asp 197 | engineered mutation |
Val 224 | Leu 224 | engineered mutation |
Asp 236 | Lys 236 | engineered mutation |
Gly 252 | Thr 252 | engineered mutation |
Asp 290 | Asn 290 | engineered mutation |
Pro 296 | Leu 296 | engineered mutation |
Ile 340 | Leu 340 | engineered mutation |
Gln 353 | Met 353 | engineered mutation |
Arg 358 | Lys 358 | engineered mutation |
Glu 360 | Leu 360 | engineered mutation |
Lys 363 | Val 363 | engineered mutation |
Asp 365 | Ala 365 | engineered mutation |
Asp 370 | Thr 370 | engineered mutation |
Phe 371 | Tyr 371 | engineered mutation |
Asp 378 | Asn 378 | engineered mutation |
Val 393 | Thr 393 | engineered mutation |
Lys 398 | Leu 398 | engineered mutation |
Phe 443 | Tyr 443 | engineered mutation |
Leu 457 | - | expression tag |
Glu 458 | - | expression tag |
His 459 | - | expression tag |
His 460 | - | expression tag |
His 461 | - | expression tag |
His 462 | - | expression tag |
His 463 | - | expression tag |
His 464 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 108262.6 | |
Non-Polymers* | Number of molecules | 15 |
Total formula weight | 4082.7 | |
All* | Total formula weight | 112345.3 |