8B0A

Cryo-EM structure of ALC1 bound to an asymmetric, site-specifically PARylated nucleosome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (K)	Chromodomain-helicase-DNA-binding protein 1-like	polymer	872	98906.8	1	UniProt (Q86WJ1) Pfam (PF00176) Pfam (PF00271)	Homo sapiens (human)	Amplified in liver cancer protein 1
2	B, F (A, E)	Histone H3	polymer	136	15403.1	2	UniProt (A0A310TTQ1) Pfam (PF00125) UniProt (by SIFTS) (P84233)	Xenopus laevis (African clawed frog)
3	C, G (B, F)	Histone H4	polymer	103	11394.4	2	UniProt (P62799) Pfam (PF15511)	Xenopus laevis (African clawed frog)
4	D, H (C, G)	Histone H2A type 1	polymer	130	14109.4	2	UniProt (P06897) Pfam (PF00125) Pfam (PF16211)	Xenopus laevis (African clawed frog)
5	E, I (D, H)	Histone H2B 1.1	polymer	123	13655.9	2	UniProt (P02281) Pfam (PF00125)	Xenopus laevis (African clawed frog)	H2B1.1
6	J (I)	DNA (149-MER) Widom 601 sequence	polymer	160	49175.3	1		synthetic construct
7	K (J)	DNA (149-MER) Widom 601 sequence	polymer	160	49606.6	1		synthetic construct

Sequence modifications

K: 16 - 879 (UniProt: Q86WJ1)

PDB	External Database	Details
Met 15	-	initiating methionine
Ala 880	-	expression tag
His 881	-	expression tag
His 882	-	expression tag
His 883	-	expression tag
His 884	-	expression tag
His 885	-	expression tag
His 886	-	expression tag

A, E: 0 - 135 (UniProt: A0A310TTQ1)

PDB	External Database	Details
Ala 110	Cys 111	engineered mutation

C, G: 0 - 129 (UniProt: P06897)

PDB	External Database	Details
Arg 99	Gly 100	conflict
Ser 123	Ala 124	conflict

D, H: 1 - 122 (UniProt: P02281)

PDB	External Database	Details
Met 0	-	initiating methionine
Thr 29	Ser 33	conflict

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	306814.4
All*	Total formula weight	306814.4

*Water molecules are not included.