8AZF

Crystal structure of K449E variant of S-adenosyl-L-homocysteine hydrolase from Pseudomonas aeruginosa in complex with adenosine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Adenosylhomocysteinase	polymer	472	51735.0	2	UniProt (B7V419) Pfam (PF05221) Pfam (PF00670) UniProt (by SIFTS) (Q9I685) In PDB	Pseudomonas aeruginosa PAO1	S-adenosyl-L-homocysteine hydrolase,AdoHcyase
2	A, C	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	2	Chemie (NAD)
3	A, C	ADENOSINE	non-polymer		267.2	2	Chemie (ADN)
4	A, C	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
5	A, C	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
6		water	water		18.0	1607	Chemie (HOH)

Sequence modifications

A, C: 1 - 469 (UniProt: B7V419)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag
Glu 449	Lys 449	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	103469.9
Non-Polymers*	Number of molecules	8
	Total formula weight	2129.5
All*	Total formula weight	105599.4

*Water molecules are not included.