8AYP
NaK C-DI mutant with Rb+ and Ba2+
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, C, D, B) | Potassium channel protein | polymer | 96 | 10735.7 | 4 | UniProt (Q81HW2) Pfam (PF07885) | Bacillus cereus ATCC 14579 | |
| 2 | AA, BA, CA, E, F... (D, A, B, C) | (4S)-2-METHYL-2,4-PENTANEDIOL | non-polymer | 118.2 | 29 | Chemie (MPD) | |||
| 3 | DA, G, H, T (D, A, C) | RUBIDIUM ION | non-polymer | 85.5 | 4 | Chemie (RB) | |||
| 4 | EA, I, J, U (D, A, C) | BARIUM ION | non-polymer | 137.3 | 4 | Chemie (BA) | |||
| 5 | PA, QA, RA, SA (A, C, D, B) | water | water | 18.0 | 72 | Chemie (HOH) |
Sequence modifications
A, C, D, B: 19 - 109 (UniProt: Q81HW2)
| PDB | External Database | Details |
|---|---|---|
| Met 18 | - | initiating methionine |
| Cys 66 | Asp 66 | engineered mutation |
| - | Gly 67 | deletion |
| Asp 67 | Asn 68 | engineered mutation |
| Ile 68 | Phe 69 | engineered mutation |
| Leu 110 | - | expression tag |
| Val 111 | - | expression tag |
| Pro 112 | - | expression tag |
| Arg 113 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 42942.8 | |
| Non-Polymers* | Number of molecules | 37 |
| Total formula weight | 4318.2 | |
| All* | Total formula weight | 47261.1 |
