8AUO

OPR3 Y370F variant in complex with 2-methoxyethyl (Z)-2-(hydroxyimino)-3-oxobutanoate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (B, A)	12-oxophytodienoate reductase 3	polymer	404	44627.5	2	UniProt (Q9FEW9) Pfam (PF00724)	Solanum lycopersicum (tomato)	12-oxophytodienoate-10,11-reductase 3,OPDA-reductase 3,LeOPR3
2	C, E (B, A)	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	2	Chemie (FMN)
3	D, F (B, A)	2-methoxyethyl (2~{Z})-2-hydroxyimino-3-oxidanylidene-butanoate	non-polymer		189.2	2	Chemie (O8R)
4	G, H (B, A)	water	water		18.0	669	Chemie (HOH)

Sequence modifications

B, A: 1 - 396 (UniProt: Q9FEW9)

PDB	External Database	Details
Phe 370	Tyr 370	engineered mutation
Leu 397	-	expression tag
Glu 398	-	expression tag
His 399	-	expression tag
His 400	-	expression tag
His 401	-	expression tag
His 402	-	expression tag
His 403	-	expression tag
His 404	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	89254.9
Non-Polymers*	Number of molecules	4
	Total formula weight	1291.0
All*	Total formula weight	90545.9

*Water molecules are not included.