8AUF
XenA Y183F variant in complex with ethyl (Z)-2-(hydroxyimino)-3-oxopentanoate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | NADH:flavin oxidoreductase | polymer | 371 | 40936.0 | 2 | UniProt (Q9R9V9) Pfam (PF00724) | Pseudomonas putida | Xenobiotic reductase,Xenobiotic reductase A |
| 2 | C, F, H (A, B) | ethyl (2~{Z})-2-hydroxyimino-3-oxidanylidene-pentanoate | non-polymer | 173.2 | 3 | Chemie (O8I) | |||
| 3 | D, G (A, B) | FLAVIN MONONUCLEOTIDE | non-polymer | 456.3 | 2 | Chemie (FMN) | |||
| 4 | E (A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 5 | I, J (A, B) | water | water | 18.0 | 992 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 363 (UniProt: Q9R9V9)
| PDB | External Database | Details |
|---|---|---|
| Phe 183 | Tyr 183 | engineered mutation |
| Leu 364 | - | expression tag |
| Glu 365 | - | expression tag |
| His 366 | - | expression tag |
| His 367 | - | expression tag |
| His 368 | - | expression tag |
| His 369 | - | expression tag |
| His 370 | - | expression tag |
| His 371 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 81872.1 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1538.3 | |
| All* | Total formula weight | 83410.4 |
