8ATT
Cryo-EM structure of yeast mitochondrial RNA polymerase transcription initiation complex with 4-mer RNA, pppGpGpUpA (IC4)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | B | Mitochondrial transcription factor 1 | polymer | 354 | 41151.2 | 1 | UniProt (P14908) Pfam (PF00398) In PDB | Saccharomyces cerevisiae S288C | Mitochondrial transcription factor mtTFB,Mitochondrial-specificity factor,RF1023 |
2 | A | DNA-directed RNA polymerase, mitochondrial | polymer | 1262 | 143282.7 | 1 | UniProt (P13433) Pfam (PF14700) Pfam (PF00940) In PDB | Saccharomyces cerevisiae S288C | |
3 | N | Non-template DNA (33-MER) | polymer | 33 | 10248.7 | 1 | Saccharomyces cerevisiae S288C | ||
4 | T | Template DNA (33-MER) | polymer | 33 | 10047.5 | 1 | Saccharomyces cerevisiae S288C | ||
5 | C | RNA (pppGpGpUpA) | polymer | 3 | 935.6 | 1 | Saccharomyces cerevisiae S288C | ||
6 | C | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) |
Sequence modifications
B: 2 - 341 (UniProt: P14908)
A: 100 - 1351 (UniProt: P13433)
PDB | External Database | Details |
---|---|---|
Met -12 | - | initiating methionine |
Gly -11 | - | expression tag |
Gly -10 | - | expression tag |
Ser -9 | - | expression tag |
His -8 | - | expression tag |
His -7 | - | expression tag |
His -6 | - | expression tag |
His -5 | - | expression tag |
His -4 | - | expression tag |
His -3 | - | expression tag |
Gly -2 | - | expression tag |
Met -1 | - | expression tag |
Ala 0 | - | expression tag |
Ser 1 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Gly 90 | - | expression tag |
Ala 91 | - | expression tag |
Met 92 | - | expression tag |
Gly 93 | - | expression tag |
Ser 94 | - | expression tag |
Gly 95 | - | expression tag |
Ile 96 | - | expression tag |
Gln 97 | - | expression tag |
Arg 98 | - | expression tag |
Pro 99 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 205665.7 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 523.2 | |
All* | Total formula weight | 206188.8 |