8AT0
PotF with mutation D247K
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Putrescine-binding periplasmic protein PotF | polymer | 350 | 39184.4 | 2 | UniProt (P31133) Pfam (PF13416) In PDB | Escherichia coli K-12 | |
2 | A, B | (2~{S})-1-(2-methoxyethoxy)propan-2-amine | non-polymer | 133.2 | 3 | Chemie (ONT) | |||
3 | A, B | SULFATE ION | non-polymer | 96.1 | 4 | Chemie (SO4) | |||
4 | A, B | (2R)-1-methoxypropan-2-amine | non-polymer | 89.1 | 5 | Chemie (JFN) | |||
5 | B | MALONATE ION | non-polymer | 102.0 | 1 | Chemie (MLI) | |||
6 | B | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
7 | water | water | 18.0 | 306 | Chemie (HOH) |
Sequence modifications
A, B: 29 - 370 (UniProt: P31133)
PDB | External Database | Details |
---|---|---|
Lys 247 | Asp 247 | engineered mutation |
Leu 371 | - | expression tag |
Glu 372 | - | expression tag |
His 373 | - | expression tag |
His 374 | - | expression tag |
His 375 | - | expression tag |
His 376 | - | expression tag |
His 377 | - | expression tag |
His 378 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 78368.9 | |
Non-Polymers* | Number of molecules | 14 |
Total formula weight | 1393.6 | |
All* | Total formula weight | 79762.5 |