8AT0

PotF with mutation D247K

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Putrescine-binding periplasmic protein PotF	polymer	350	39184.4	2	UniProt (P31133) Pfam (PF13416)	Escherichia coli K-12
2	C, D, L (A, B)	(2~{S})-1-(2-methoxyethoxy)propan-2-amine	non-polymer		133.2	3	Chemie (ONT)
3	E, F, N, O (A, B)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
4	G, H, I, J, P (A, B)	(2R)-1-methoxypropan-2-amine	non-polymer		89.1	5	Chemie (JFN)
5	K (B)	MALONATE ION	non-polymer		102.0	1	Chemie (MLI)
6	M (B)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
7	Q, R (A, B)	water	water		18.0	306	Chemie (HOH)

Sequence modifications

A, B: 29 - 370 (UniProt: P31133)

PDB	External Database	Details
Lys 247	Asp 247	engineered mutation
Leu 371	-	expression tag
Glu 372	-	expression tag
His 373	-	expression tag
His 374	-	expression tag
His 375	-	expression tag
His 376	-	expression tag
His 377	-	expression tag
His 378	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	78368.9
Non-Polymers*	Number of molecules	14
	Total formula weight	1393.6
All*	Total formula weight	79762.5

*Water molecules are not included.