8ASZ

PotF with mutations S87Y and A182D in complex with Agmatine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Putrescine-binding periplasmic protein PotF	polymer	352	39490.6	1	UniProt (P31133) Pfam (PF13416)	Escherichia coli K-12
2	B (A)	AGMATINE	non-polymer		130.2	1	Chemie (AG2)
3	C, H, I, J, K... (A)	1,2-ETHANEDIOL	non-polymer		62.1	10	Chemie (EDO)
4	D, E (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	F, G (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
6	Q (A)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
7	R (A)	water	water		18.0	355	Chemie (HOH)

Sequence modifications

A: 27 - 370 (UniProt: P31133)

PDB	External Database	Details
Tyr 87	Ser 87	engineered mutation
Asp 182	Ala 182	engineered mutation
Leu 371	-	expression tag
Glu 372	-	expression tag
His 373	-	expression tag
His 374	-	expression tag
His 375	-	expression tag
His 376	-	expression tag
His 377	-	expression tag
His 378	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	39490.6
Non-Polymers*	Number of molecules	16
	Total formula weight	1187.4
All*	Total formula weight	40678.0

*Water molecules are not included.