8AQO
Streptavidin with a bisbiothinilated Fe4S4 cluster
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Streptavidin | polymer | 158 | 16364.7 | 4 | UniProt (P22629) Pfam (PF01382) | Streptomyces avidinii | |
| 2 | E, F (A, C) | 5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-[2-[[20-[2-[5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethanoylamino]-2$l^{3},4,12,14$l^{3},16,24,25$l^{3},27$l^{3}-octathia-1$l^{4},3$l^{4},13$l^{4},15$l^{4}-tetraferranonacyclo[11.11.1.1^{1,13}.1^{6,10}.1^{18,22}.0^{2,15}.0^{3,14}.0^{3,25}.0^{15,27}]octacosa-6(28),7,9,18,20,22(26)-hexaen-8-yl]amino]-2-oxidanylidene-ethyl]pentanamide | non-polymer | 1284.9 | 2 | Chemie (NUI) | |||
| 3 | G, H, I, J (A, B, C, D) | water | water | 18.0 | 144 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 14 - 159 (UniProt: P22629)
| PDB | External Database | Details |
|---|---|---|
| Ala 2 | - | expression tag |
| Ser 3 | - | expression tag |
| Met 4 | - | expression tag |
| Thr 5 | - | expression tag |
| Gly 6 | - | expression tag |
| Gly 7 | - | expression tag |
| Gln 8 | - | expression tag |
| Gln 9 | - | expression tag |
| Met 10 | - | expression tag |
| Gly 11 | - | expression tag |
| Arg 12 | - | expression tag |
| Asp 13 | - | expression tag |
| Ala 112 | Ser 136 | engineered mutation |
| Ala 121 | Lys 145 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 65458.9 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 2569.8 | |
| All* | Total formula weight | 68028.7 |
