8AQ6
NanoLuc luciferase with bound furimamide in surface allosteric site
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D, E... | NanoLuc luciferase | polymer | 181 | 20389.2 | 8 | UniProt (Q9GV45) In PDB | Oplophorus gracilirostris (Luminous shrimp) | 19kOLase,Oplophorus-luciferin 2-monooxygenase catalytic subunit |
2 | A, H | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
3 | E, A, B, C | TETRAETHYLENE GLYCOL | non-polymer | 194.2 | 7 | Chemie (PG4) | |||
4 | A | OXYGEN MOLECULE | non-polymer | 32.0 | 1 | Chemie (OXY) | |||
5 | E, F, G, A, H... | CHLORIDE ION | non-polymer | 35.5 | 22 | Chemie (CL) | |||
6 | D | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 1 | Chemie (PGE) | |||
7 | G | N-(3-Benzyl-5-phenylpyrazin-2-yl)-2-(furan-2-yl)acetamide | non-polymer | 369.4 | 1 | Chemie (NT0) | |||
8 | water | water | 18.0 | 770 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E, F, G, H: 1 - 169 (UniProt: Q9GV45)
PDB | External Database | Details |
---|---|---|
Met -11 | - | initiating methionine |
His -10 | - | expression tag |
His -9 | - | expression tag |
His -8 | - | expression tag |
His -7 | - | expression tag |
His -6 | - | expression tag |
His -5 | - | expression tag |
Ser -4 | - | expression tag |
Asp -3 | - | expression tag |
Asn -2 | - | expression tag |
Met -1 | - | expression tag |
Val 0 | - | expression tag |
Glu 4 | Ala 31 | engineered mutation |
Arg 11 | Gln 38 | engineered mutation |
Leu 18 | Gln 45 | engineered mutation |
Val 27 | Leu 54 | engineered mutation |
Asn 33 | Ala 60 | engineered mutation |
Arg 43 | Lys 70 | engineered mutation |
Ile 44 | Val 71 | engineered mutation |
Ile 54 | Ala 81 | engineered mutation |
Asp 68 | Phe 95 | engineered mutation |
Gln 72 | Leu 99 | engineered mutation |
Lys 75 | Met 102 | engineered mutation |
Val 90 | Ile 117 | engineered mutation |
Glu 115 | Pro 142 | engineered mutation |
Lys 124 | Gln 151 | engineered mutation |
Ile 138 | Tyr 165 | engineered mutation |
Arg 166 | Asn 193 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 8 |
Total formula weight | 163113.9 | |
Non-Polymers* | Number of molecules | 34 |
Total formula weight | 2875.3 | |
All* | Total formula weight | 165989.2 |