8AJT
Crystal structure of the H323A mutant of S-adenosyl-L-homocysteine hydrolase from Pseudomonas aeruginosa cocrystallized with adenosine in the presence of K+ cations
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | AAA, BBB, CCC, DDD | Adenosylhomocysteinase | polymer | 472 | 51668.0 | 4 | UniProt (Q9I685) Pfam (PF05221) Pfam (PF00670) In PDB | Pseudomonas aeruginosa PAO1 | S-adenosyl-L-homocysteine hydrolase,AdoHcyase |
| 2 | AAA, DDD, BBB, CCC | ADENOSINE | non-polymer | 267.2 | 4 | Chemie (ADN) | |||
| 3 | AAA, DDD, BBB, CCC | NICOTINAMIDE-ADENINE-DINUCLEOTIDE | non-polymer | 663.4 | 4 | Chemie (NAD) | |||
| 4 | AAA, DDD, BBB, CCC | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 8 | Chemie (TRS) | |||
| 5 | CCC, DDD, AAA | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 4 | Chemie (PEG) | |||
| 6 | CCC, AAA, DDD, BBB | POTASSIUM ION | non-polymer | 39.1 | 5 | Chemie (K) | |||
| 7 | CCC, AAA, DDD | PHOSPHATE ION | non-polymer | 95.0 | 4 | Chemie (PO4) | |||
| 8 | CCC, AAA, DDD, BBB | CHLORIDE ION | non-polymer | 35.5 | 10 | Chemie (CL) | |||
| 9 | water | water | 18.0 | 1575 | Chemie (HOH) |
Sequence modifications
AAA, BBB, CCC, DDD: 1 - 469 (UniProt: Q9I685)
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Ala 323 | His 323 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 206671.9 | |
| Non-Polymers* | Number of molecules | 39 |
| Total formula weight | 6054.2 | |
| All* | Total formula weight | 212726.1 |
