8AI6

Crystal structure of radical SAM epimerase EpeE D210A mutant from Bacillus subtilis with [4Fe-4S] clusters, S-adenosyl-L-homocysteine and persulfurated cysteine bound

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Putative peptide biosynthesis protein YydG	polymer	344	39940.5	2	UniProt (Q45595) Pfam (PF13353) Pfam (PF04055)	Bacillus subtilis
2	C, D, K, L (A, B)	IRON/SULFUR CLUSTER	non-polymer		351.6	4	Chemie (SF4)
3	E, M (A, B)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	2	Chemie (SAH)
4	AA, BA, CA, F, G... (B, A)	1,2-ETHANEDIOL	non-polymer		62.1	18	Chemie (EDO)
5	I, U, Y (A, B)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)
6	DA, EA (A, B)	water	water		18.0	387	Chemie (HOH)

Sequence modifications

A, B: 1 - 319 (UniProt: Q45595)

PDB	External Database	Details
Met -24	-	initiating methionine
Ala -23	-	expression tag
Ser -22	-	expression tag
Trp -21	-	expression tag
Ser -20	-	expression tag
His -19	-	expression tag
Pro -18	-	expression tag
Gln -17	-	expression tag
Phe -16	-	expression tag
Glu -15	-	expression tag
Lys -14	-	expression tag
Ser -13	-	expression tag
Gly -12	-	expression tag
Gly -11	-	expression tag
Gly -10	-	expression tag
Gly -9	-	expression tag
Gly -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag
Ala 210	Asp 210	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	79881.0
Non-Polymers*	Number of molecules	27
	Total formula weight	3611.0
All*	Total formula weight	83492.0

*Water molecules are not included.