8AGE
Structure of yeast oligosaccharylransferase complex with acceptor peptide bound
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 | polymer | 718 | 81604.5 | 1 | UniProt (P39007) Pfam (PF02516) Pfam (PF21436) | Saccharomyces cerevisiae (baker's yeast) | Oligosaccharyl transferase subunit STT3,Staurosporine and temperature sensitivity protein 3 |
| 2 | B (B) | OST4 isoform 1 | polymer | 65 | 6871.8 | 1 | UniProt (A0A8H8UM72) Pfam (PF10215) UniProt (by SIFTS) (Q99380) | Saccharomyces cerevisiae (baker's yeast) | |
| 3 | C (C) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5 | polymer | 86 | 9525.1 | 1 | UniProt (Q92316) Pfam (PF05251) | Saccharomyces cerevisiae (baker's yeast) | Oligosaccharyl transferase subunit OST5,Oligosaccharyl transferase 9.5 kDa subunit,Oligosaccharyl transferase subunit zeta |
| 4 | D (D) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2 | polymer | 130 | 14712.5 | 1 | UniProt (A0A8H4BUV6) Pfam (PF02109) UniProt (by SIFTS) (P46964) | Saccharomyces cerevisiae (baker's yeast) | Oligosaccharyl transferase subunit OST2 |
| 5 | E (E) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 | polymer | 476 | 54116.5 | 1 | UniProt (A0A6A5PXA1) Pfam (PF04597) UniProt (by SIFTS) (P41543) | Saccharomyces cerevisiae (baker's yeast) | |
| 6 | F (F) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 | polymer | 283 | 31340.4 | 1 | UniProt (A0A6V8S2Y6) Pfam (PF05817) UniProt (by SIFTS) (Q02795) | Saccharomyces cerevisiae (baker's yeast) | |
| 7 | G (G) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 | polymer | 430 | 49444.4 | 1 | UniProt (A0A8H8ULL1) Pfam (PF03345) UniProt (by SIFTS) (P33767) | Saccharomyces cerevisiae (baker's yeast) | Oligosaccharyl transferase subunit WBP1 |
| 8 | H (H) | OST3 isoform 1 | polymer | 350 | 39518.2 | 1 | UniProt (A0A6A5Q3S9) Pfam (PF04756) UniProt (by SIFTS) (P48439) | Saccharomyces cerevisiae (baker's yeast) | |
| 9 | I (P) | PEPTIDE | polymer | 7 | 696.7 | 1 | Saccharomyces cerevisiae | ||
| 10 | J (K) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1235.1 | 1 | In PDB GlyTouCan (G46836GH) | |||
| 11 | K, L (I, J) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 2 | In PDB GlyTouCan (G42666HT) | |||
| 12 | M (A) | MANGANESE (II) ION | non-polymer | 54.9 | 1 | Chemie (MN) | |||
| 13 | N, U, V, W (A, B, E, F) | 1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE | non-polymer | 758.1 | 4 | Chemie (CPL) | |||
| 14 | O (A) | PHOSPHATIDYLETHANOLAMINE | non-polymer | 734.0 | 1 | Chemie (PTY) | |||
| 15 | BA, P, Q, R, S... (G, A, B, F) | (2~{S},3~{R},4~{R},5~{S},6~{S})-2-(hydroxymethyl)-6-[(1~{S},2~{R},3~{R},4~{R},5'~{S},6~{S},7~{R},8~{S},9~{R},12~{R},13~{R},15~{S},16~{S},18~{R})-5',7,9,13-tetramethyl-3,15-bis(oxidanyl)spiro[5-oxapentacyclo[10.8.0.0^{2,9}.0^{4,8}.0^{13,18}]icosane-6,2'-oxane]-16-yl]oxy-oxane-3,4,5-triol | non-polymer | 610.8 | 8 | Chemie (KZB) | |||
| 16 | AA, Z (G) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 2 | Chemie (NAG) | |||
| 17 | CA (P) | 2-[3,6-bis(dimethylamino)xanthen-9-yl]-5-methanoyl-benzoate | non-polymer | 414.5 | 1 | Chemie (323) | |||
| 18 | DA, EA, FA, GA (A, C, E, G) | water | water | 18.0 | 9 | Chemie (HOH) |
Sequence modifications
B: 1 - 36 (UniProt: A0A8H8UM72)
| PDB | External Database | Details |
|---|---|---|
| Arg 37 | - | expression tag |
| Thr 38 | - | expression tag |
| Leu 39 | - | expression tag |
| Gln 40 | - | expression tag |
| Val 41 | - | expression tag |
| Asp 42 | - | expression tag |
| Gly 43 | - | expression tag |
| Gly 44 | - | expression tag |
| Ser 45 | - | expression tag |
| Gly 46 | - | expression tag |
| Gly 47 | - | expression tag |
| Ser 48 | - | expression tag |
| Leu 49 | - | expression tag |
| Glu 50 | - | expression tag |
| Val 51 | - | expression tag |
| Leu 52 | - | expression tag |
| Phe 53 | - | expression tag |
| Gln 54 | - | expression tag |
| Gly 55 | - | expression tag |
| Pro 56 | - | expression tag |
| Thr 57 | - | expression tag |
| Glu 58 | - | expression tag |
| Thr 59 | - | expression tag |
| Ser 60 | - | expression tag |
| Gln 61 | - | expression tag |
| Val 62 | - | expression tag |
| Ala 63 | - | expression tag |
| Pro 64 | - | expression tag |
| Ala 65 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 287830.2 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 2083.9 | |
| Non-Polymers* | Number of molecules | 17 |
| Total formula weight | 9564.3 | |
| All* | Total formula weight | 299478.4 |
