8AGB
Structure of yeast oligosaccharylransferase complex with lipid-linked oligosaccharide bound
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 | polymer | 718 | 81604.5 | 1 | UniProt (P39007) Pfam (PF02516) Pfam (PF21436) | Saccharomyces cerevisiae (baker's yeast) | Oligosaccharyl transferase subunit STT3,Staurosporine and temperature sensitivity protein 3 |
| 2 | B (B) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4 | polymer | 65 | 6871.8 | 1 | UniProt (Q99380) Pfam (PF10215) | Saccharomyces cerevisiae (baker's yeast) | Oligosaccharyl transferase subunit OST4,Oligosaccharyl transferase 4 kDa subunit,OTase 4 kDa subunit |
| 3 | C (C) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5 | polymer | 86 | 9525.1 | 1 | UniProt (Q92316) Pfam (PF05251) | Saccharomyces cerevisiae (baker's yeast) | Oligosaccharyl transferase subunit OST5,Oligosaccharyl transferase 9.5 kDa subunit,Oligosaccharyl transferase subunit zeta |
| 4 | D (D) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2 | polymer | 130 | 14712.5 | 1 | UniProt (P46964) Pfam (PF02109) | Saccharomyces cerevisiae (baker's yeast) | Oligosaccharyl transferase subunit OST2,Oligosaccharyl transferase 16 kDa subunit,Oligosaccharyl transferase subunit epsilon |
| 5 | E (E) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 | polymer | 476 | 54116.5 | 1 | UniProt (P41543) Pfam (PF04597) | Saccharomyces cerevisiae (baker's yeast) | N-linked oligosaccharyl transferase subunit 1,Oligosaccharyl transferase 64 kDa subunit,Oligosaccharyl transferase subunit OST1,Oligosaccharyl transferase subunit alpha |
| 6 | F (F) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1 | polymer | 285 | 31554.7 | 1 | UniProt (Q02795) Pfam (PF25147) | Saccharomyces cerevisiae (baker's yeast) | Oligosaccharyl transferase subunit SWP1,Oligosaccharyl transferase subunit delta |
| 7 | G (G) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 | polymer | 430 | 49444.4 | 1 | UniProt (P33767) Pfam (PF03345) Pfam (PF23358) | Saccharomyces cerevisiae (baker's yeast) | Oligosaccharyl transferase subunit WBP1,Oligosaccharyl transferase subunit beta |
| 8 | H (H) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3 | polymer | 350 | 39518.2 | 1 | UniProt (P48439) Pfam (PF04756) | Saccharomyces cerevisiae (baker's yeast) | Oligosaccharyl transferase 34 kDa subunit,Oligosaccharyl transferase subunit OST3,Oligosaccharyl transferase subunit gamma |
| 9 | I (I) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1235.1 | 1 | In PDB GlyTouCan (G46836GH) | |||
| 10 | J (J) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 1 | In PDB GlyTouCan (G15407YE) | |||
| 11 | K (K) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
| 12 | L (L) | alpha-D-glucopyranose-(1-2)-alpha-D-glucopyranose-(1-3)-alpha-D-glucopyranose-(1-3)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-glucopyranose | branched | 1883.7 | 1 | In PDB | |||
| 13 | M, Q, R, V (A, B, E, G) | 1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE | non-polymer | 758.1 | 4 | Chemie (CPL) | |||
| 14 | N, S (A, F) | PHOSPHATIDYLETHANOLAMINE | non-polymer | 734.0 | 2 | Chemie (PTY) | |||
| 15 | O (A) | MANGANESE (II) ION | non-polymer | 54.9 | 1 | Chemie (MN) | |||
| 16 | P (A) | phosphono [(3~{R},6~{E},10~{E})-3,7,11,15-tetramethylhexadeca-6,10,14-trienyl] hydrogen phosphate | non-polymer | 452.5 | 1 | Chemie (ELU) | |||
| 17 | T, U (G) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 2 | Chemie (NAG) |
Sequence modifications
B: 1 - 36 (UniProt: Q99380)
F: 2 - 284 (UniProt: Q02795)
| PDB | External Database | Details |
|---|---|---|
| Arg 37 | - | expression tag |
| Thr 38 | - | expression tag |
| Leu 39 | - | expression tag |
| Gln 40 | - | expression tag |
| Val 41 | - | expression tag |
| Asp 42 | - | expression tag |
| Gly 43 | - | expression tag |
| Gly 44 | - | expression tag |
| Ser 45 | - | expression tag |
| Gly 46 | - | expression tag |
| Gly 47 | - | expression tag |
| Ser 48 | - | expression tag |
| Leu 49 | - | expression tag |
| Glu 50 | - | expression tag |
| Val 51 | - | expression tag |
| Leu 52 | - | expression tag |
| Phe 53 | - | expression tag |
| Gln 54 | - | expression tag |
| Gly 55 | - | expression tag |
| Pro 56 | - | expression tag |
| Thr 57 | - | expression tag |
| Glu 58 | - | expression tag |
| Thr 59 | - | expression tag |
| Ser 60 | - | expression tag |
| Gln 61 | - | expression tag |
| Val 62 | - | expression tag |
| Ala 63 | - | expression tag |
| Pro 64 | - | expression tag |
| Ala 65 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Thr 285 | - | expression tag |
| Ile 286 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 287347.8 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 4129.7 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 5450.1 | |
| All* | Total formula weight | 296927.6 |
