8AFV

DaArgC3 - Engineered Formyl Phosphate Reductase with 3 substitutions (S178V, G182V, L233I)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	N-acetyl-gamma-glutamyl-phosphate reductase	polymer	342	38001.5	4	UniProt (D4H3H4) Pfam (PF01118) Pfam (PF22698)	Denitrovibrio acetiphilus DSM 12809	AGPR,N-acetyl-glutamate semialdehyde dehydrogenase,NAGSA dehydrogenase
2	E, F, G, H (A, B, C)	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
3	I, J, K, L (A, B, C, D)	water	water		18.0	534	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 334 (UniProt: D4H3H4)

PDB	External Database	Details
Val 178	Ser 178	engineered mutation
Val 182	Gly 182	engineered mutation
Ile 233	Leu 233	engineered mutation
Leu 335	-	expression tag
Glu 336	-	expression tag
His 337	-	expression tag
His 338	-	expression tag
His 339	-	expression tag
His 340	-	expression tag
His 341	-	expression tag
His 342	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	152005.9
Non-Polymers*	Number of molecules	4
	Total formula weight	92.0
All*	Total formula weight	152097.8

*Water molecules are not included.