8AFK

Structure of iRFP variant C15S/N136R/V256C in complex with phycocyanobilin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Near-infrared fluorescent protein	polymer	316	34671.7	2	UniProt (G1FNL7) Pfam (PF08446) Pfam (PF01590)	Rhodopseudomonas palustris CGA009
2	C, D (A, B)	PHYCOCYANOBILIN	non-polymer		588.7	2	Chemie (CYC)
3	E, F (A, B)	water	water		18.0	134	Chemie (HOH)

Sequence modifications

A, B: 1 - 316 (UniProt: G1FNL7)

PDB	External Database	Details
Ser 15	Cys 15	engineered mutation
Arg 136	Asn 136	engineered mutation
Cys 254	Val 254	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	69343.4
Non-Polymers*	Number of molecules	2
	Total formula weight	1177.4
All*	Total formula weight	70520.7

*Water molecules are not included.