8AFJ
tRNA modifying enzyme MiaE soaked in Na-dithionite in a glovebox and flash-cooled using a miniature-airlock
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (B, A) | tRNA hydroxylase | polymer | 200 | 22661.1 | 2 | UniProt (A0A179QS89) Pfam (PF06175) | Pseudomonas putida KT2440 | tRNA-(MS[2]IO[6]A)-hydroxylase (MiaE),tRNA-(Ms[2]io[6]A)-hydroxylase |
| 2 | C, D, K, L (B, A) | FE (III) ION | non-polymer | 55.8 | 4 | Chemie (FE) | |||
| 3 | E, M (B, A) | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 2 | Chemie (TRS) | |||
| 4 | F, N, O (B, A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 3 | Chemie (PEG) | |||
| 5 | G (B) | PENTAETHYLENE GLYCOL | non-polymer | 238.3 | 1 | Chemie (1PE) | |||
| 6 | H, I, P (B, A) | CALCIUM ION | non-polymer | 40.1 | 3 | Chemie (CA) | |||
| 7 | J (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 8 | Q, R (B, A) | water | water | 18.0 | 249 | Chemie (HOH) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 45322.2 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 1180.0 | |
| All* | Total formula weight | 46502.1 |
