8ADI

Cryo-EM structure of Darobactin 9 bound BAM complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Outer membrane protein assembly factor BamA	polymer	813	90918.7	1	UniProt (P0A940) Pfam (PF07244) Pfam (PF01103) In PDB	Escherichia coli	Omp85
2	B	Outer membrane protein assembly factor BamB	polymer	405	43478.8	1	UniProt (P77774) Pfam (PF01011) Pfam (PF13360) In PDB	Escherichia coli
3	C	Outer membrane protein assembly factor BamC	polymer	347	37150.6	1	UniProt (P0A903) Pfam (PF06804) In PDB	Escherichia coli
4	D	Outer membrane protein assembly factor BamD	polymer	248	28133.7	1	UniProt (P0AC02) Pfam (PF13525) In PDB	Escherichia coli K-12
5	E	Outer membrane protein assembly factor BamE	polymer	124	13657.5	1	UniProt (P0A937) Pfam (PF04355) In PDB	Escherichia coli
6	F	Darobactin 9	polymer	7	1010.1	1	BIRD (PRD_002430)	synthetic construct

Sequence modifications

A: 1 - 810 (UniProt: P0A940)

PDB	External Database	Details
Met -2	-	initiating methionine
Gly -1	-	expression tag
Ser 0	-	expression tag

B: 1 - 392 (UniProt: P77774)

PDB	External Database	Details
Met -2	-	initiating methionine
Gly -1	-	expression tag
Ser 0	-	expression tag
Lys 393	-	expression tag
Leu 394	-	expression tag
Trp 395	-	expression tag
Ser 396	-	expression tag
His 397	-	expression tag
Pro 398	-	expression tag
Gln 399	-	expression tag
Phe 400	-	expression tag
Glu 401	-	expression tag
Lys 402	-	expression tag

C: 1 - 344 (UniProt: P0A903)

PDB	External Database	Details
Met -2	-	initiating methionine
Gly -1	-	expression tag
Ser 0	-	expression tag

D: 1 - 245 (UniProt: P0AC02)

PDB	External Database	Details
Met -2	-	initiating methionine
Gly -1	-	expression tag
Ser 0	-	expression tag

E: 1 - 113 (UniProt: P0A937)

PDB	External Database	Details
Met -2	-	initiating methionine
Gly -1	-	expression tag
Ser 0	-	expression tag
Lys 114	-	expression tag
Leu 115	-	expression tag
His 116	-	expression tag
His 117	-	expression tag
His 118	-	expression tag
His 119	-	expression tag
His 120	-	expression tag
His 121	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	214349.3
All*	Total formula weight	214349.3

*Water molecules are not included.